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Proteomic profiles ...
Proteomic profiles in cerebrospinal fluid predicted death and disability in term infants with perinatal asphyxia : A pilot study
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- Leifsdottir, K. (författare)
- Karolinska Institutet
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- Thelin, E. P. (författare)
- Karolinska Institutet
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Lassarén, P. (författare)
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- Siljehav, V. (författare)
- Karolinska Institutet
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- Nilsson, Peter (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Eksborg, S. (författare)
- Karolinska Institutet
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- Herlenius, E. (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2022-02-17
- 2022
- Engelska.
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Ingår i: Acta Paediatrica. - : Wiley. - 0803-5253 .- 1651-2227. ; 111:5, s. 961-970
- Relaterad länk:
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https://doi.org/10.1...
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https://onlinelibrar...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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http://kipublication...
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Abstract
Ämnesord
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- Aim: Perinatal asphyxia, resulting in hypoxic-ischaemic encephalopathy (HIE), has been associated with high mortality rates and severe lifelong neurodevelopmental disabilities. Our aim was to study the association between the proteomic profile in cerebrospinal fluid (CSF) and the degree of HIE and long-term outcomes. Methods: We prospectively enrolled 18-term born infants with HIE and 10-term born controls between 2000 and 2004 from the Karolinska University Hospital. An antibody suspension bead array and FlexMap3D analysis was used to characterise 178 unique brain-derived and inflammation associated proteins in their CSF. Results: Increased CSF concentrations of several brain-specific proteins were observed in the proteome of HIE patients compared with the controls. An upregulation of neuroinflammatory pathways was also noted and this was confirmed by pathway analysis. Principal component analysis revealed a gradient from favourable to unfavourable HIE grades and outcomes. The proteins that provided strong predictors were structural proteins, including myelin basic protein and alpha-II spectrin. The functional proteins included energy-related proteins like neuron-specific enolase and synaptic regulatory proteins. Increased CSF levels of 51 proteins correlated with adverse outcomes in infants with HIE. Conclusion: Brain-specific proteins and neuroinflammatory mediators in CSF may predict HIE degrees and outcomes after perinatal asphyxia.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Pediatrik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Pediatrics (hsv//eng)
Nyckelord
- biomarkers
- cerebrospinal fluid
- hypoxic-ischaemic encephalopathy
- perinatal asphyxia
- protein profile
- alpha2 spectrin
- brain protein
- myelin basic protein
- neuron specific enolase
- proteome
- regulator protein
- spectrin
- structural protein
- synaptic regulatory protein
- unclassified drug
- adverse outcome
- Article
- child death
- clinical article
- clinical outcome
- controlled study
- disability
- female
- human
- hypoxic ischemic encephalopathy
- infant
- male
- pathway analysis
- pilot study
- principal component analysis
- prospective study
- protein cerebrospinal fluid level
- protein fingerprinting
- protein microarray
- upregulation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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