Turning food waste to antibacterial and biocompatible fungal chitin/chitosan monofilaments

• Here, cell wall of a zygomycete fungus, Rhizopus delemar, grown on bread waste was wet spun into monofilaments. Using the whole cell wall material omits the common chitosan isolation and purification steps and leads to higher material utilization. The fungal cell wall contained 36.9% and 19.7% chitosan and chitin, respectively. Solid state NMR of the fungal cell wall material confirmed the presence of chitosan, chitin, and other carbohydrates. Hydrogels were prepared by ultrafine grinding of the cell wall, followed by addition of lactic acid to protonate the amino groups of chitosan, and subsequently wet spun into monofilaments. The monofilament inhibited the growth of Bacillus megaterium (Gram+ bacterium) and Escherichia coli (Gram- bacterium) significantly (92.2% and 99.7% respectively). Cytotoxicity was evaluated using an in vitro assay with human dermal fibroblasts, indicating no toxic inducement from exposure of the monofilaments. The antimicrobial and biocompatible fungal monofilaments, open new avenues for sustainable biomedical textiles from abundant food waste. 

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)

NATURVETENSKAP  -- Kemi -- Organisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Organic Chemistry (hsv//eng)

NATURVETENSKAP  -- Biologi -- Mikrobiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Microbiology (hsv//eng)

Nyckelord

Antibacterial

Biocompatibility

MAS NMR

Chitin/chitosan

Fungal textiles

Wet spinning

carbohydrate

chitin

chitosan

glucosamine

glucuronic acid

hydrogel

lactic acid

n acetylglucosamine

sulfuric acid

antiinfective agent

antibacterial activity

antibiotic sensitivity

Article

Bacillus megaterium

bacterial growth

biocompatibility

cell culture

cell proliferation

cell viability

cytotoxicity

Escherichia coli

fibroblast

food waste

fungal cell wall

grinding

high performance liquid chromatography

in vitro study

ion exchange chromatography

monofilament

MTT assay

nonhuman

nuclear magnetic resonance

Rhizopus delemar

solid state

thin filament

time-lapse microscopy

viscosity

chemistry

food

human

waste disposal

Anti-Bacterial Agents

Humans

Refuse Disposal

Resource Recovery

Antibacterial

Biocompatibility

MAS NMR

Chitin/chitosan

Fungal textiles

Wet spinning

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)