A portable and low-cost centrifugal microfluidic platform for multiplexed colorimetric detection of protein biomarkers

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Akhtar, Ahmad SaleemKTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab (author)

A portable and low-cost centrifugal microfluidic platform for multiplexed colorimetric detection of protein biomarkers

Article/chapterEnglish2023

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Elsevier BV,2023
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LIBRIS-ID:oai:DiVA.org:kth-323338
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-323338URI
https://doi.org/10.1016/j.aca.2023.340823DOI
http://kipublications.ki.se/Default.aspx?queryparsed=id:151784113URI

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Language:English
Summary in:English

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SwePubSwePub

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

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QC 20230307
Cytokines play a very important role in our immune system by acting as mediators to put up a coordinated defense against foreign elements in our body. Elevated levels of cytokines in the body can signal to an ongoing response of the immune system to some abnormality. Thus, the quantification of a panel of cytokines can provide valuable information regarding the diagnosis of specific diseases and state of overall health of an individual. Conventional Enzyme Linked Immunosorbent Assay (ELISA) is the gold-standard for quantification of cytokines, however the need for trained personnel and expensive equipment limits its application to centralized laboratories only. In this context, there is a lack of simple, low-cost and portable devices which can allow for quantification of panels of cytokines at point-of-care and/or resource limited settings.Here, we report the development of a versatile, low-cost and portable bead-based centrifugal microfluidic platform allowing for multiplexed detection of cytokines with minimal hands-on time and an integrated colorimetric signal readout without the need for any external equipment. As a model, multiplexed colorimetric quantification of three target cytokines i.e., Tumor necrosis factor alpha (TNF-α), Interferon gamma (IFN-γ) and Interleukin-2 (IL-2) was achieved in less than 30 min with limits of detection in ng/mL range. The developed platform was further evaluated using spiked-in plasma samples to test for matrix interference. The ease of use, low-cost and portability of the developed platform highlight its potential to serve as a sample-to-answer solution for detection of cytokine panels in resource limited settings.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
Lab-on-a-disc
Photodetectors
Immunoassay
Cytokines
Point-of-care
Resource limited settings
Teknik och hälsa
Technology and Health

Added entries (persons, corporate bodies, meetings, titles ...)

Soares, Ruben R. G.KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi(Swepub:kth)u166jk6w (author)
Pinto, Ines FernandesKTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1g105sd (author)
Russom, Aman,Prof.1976-KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi,Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES(Swepub:kth)u1go6jxm (author)
KTHNanobioteknologi (creator_code:org_t)

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In:Analytica Chimica Acta: Elsevier BV12450003-26701873-4324

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By the university: Royal Institute of Technology; Karolinska Institutet

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