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Fully automated cen...
Fully automated centrifugal microfluidic platform for COVID-19 detection using computer vision-based readout
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- Akhtar, Ahmad Saleem (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Lapins, Noa (författare)
- KTH,Nanobioteknologi
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- Moura, João Martinho (författare)
- International Iberian Nanotechnology Laboratory
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- Paula, Luis (författare)
- International Iberian Nanotechnology Laboratory
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- Pedro, Adriano (författare)
- International Iberian Nanotechnology Laboratory
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- Martins, Fabio (författare)
- International Iberian Nanotechnology Laboratory
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- Mota, Duarte (författare)
- International Iberian Nanotechnology Laboratory
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- Pinto, Ines Fernandes (författare)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Martins, Marco (författare)
- International Iberian Nanotechnology Laboratory
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- Russom, Aman, Prof. 1976- (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- COVID-19 pandemic made it evident that the world is unprepared for effectively tackling a pandemic resulting from an infectious disease. The conventional diagnostic methods for detection of infectious diseases were limited to centralized laboratories. As the burden of testing increased with the spread of the disease, the centralized testing facilities were strained for resources and personnel. These problems were further exacerbated in low- and middle-income countries where the health and transport infrastructure are not very well developed. To overcome this reliance on centralized testing and to facilitate decentralized testing, focus was shifted towards development of novel point-of-care diagnostic methods. We report the development of a fully automated centrifugal microfluidic platform that uses loop mediated isothermal amplification (LAMP) combined with computer vision-based readout for COVID-19 detection. The integrated platform allows sample to answer analysis at the push of a single button and can process 26 samples in 40 minutes. The platform performs a completely automated assay protocol involving heating, rotation and detection without the need for user intervention. A limit of detection of approximately 100 RNA copies in 10 µL reaction was achieved using RNA fragments spiked in water and similar results were obtained for artificial saliva samples.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Biotechnology
- Bioteknologi
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)
- Av författaren/redakt...
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Akhtar, Ahmad Sa ...
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Lapins, Noa
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Moura, João Mart ...
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Paula, Luis
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Pedro, Adriano
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Martins, Fabio
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visa fler...
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Mota, Duarte
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Pinto, Ines Fern ...
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Martins, Marco
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Russom, Aman, Pr ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Medicinsk biotek ...
- Av lärosätet
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Kungliga Tekniska Högskolan