SwePub
Sök i LIBRIS databas

  Extended search

WFRF:(Joshua Douglas)
 

Search: WFRF:(Joshua Douglas) > (2020-2023) > Distinct cervical t...

  • Edfeldt, GabriellaKarolinska Institutet (author)

Distinct cervical tissue-adherent and luminal microbiome communities correlate with mucosal host gene expression and protein levels in Kenyan sex workers

  • Article/chapterEnglish2023

Publisher, publication year, extent ...

  • 2023-03-31
  • Springer Nature,2023
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:kth-326486
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-326486URI
  • https://doi.org/10.1186/s40168-023-01502-4DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-216710URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:152325704URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • QC 20230503
  • Background The majority of studies characterizing female genital tract microbiota have focused on luminal organisms, while the presence and impact of tissue-adherent ectocervical microbiota remain incompletely understood. Studies of luminal and tissue-associated bacteria in the gastrointestinal tract suggest that these communities may have distinct roles in health and disease. Here, we performed a multi-omics characterization of paired luminal and tissue samples collected from a cohort of Kenyan female sex workers.Results We identified a tissue-adherent bacterial microbiome, with a higher alpha diversity than the luminal microbiome, in which dominant genera overall included Gardnerella and Lactobacillus, followed by Prevotella, Atopobium, and Sneathia. About half of the L. iners-dominated luminal samples had a corresponding Gardnerella-dominated tissue microbiome. Broadly, the tissue-adherent microbiome was associated with fewer differentially expressed host genes than the luminal microbiome. Gene set enrichment analysis revealed that L. crispatus-dominated tissue-adherent communities were associated with protein translation and antimicrobial activity, whereas a highly diverse microbial community was associated with epithelial remodeling and pro-inflammatory pathways. Tissue-adherent communities dominated by L. iners and Gardnerella were associated with lower host transcriptional activity. Tissue-adherent microbiomes dominated by Lactobacillus and Gardnerella correlated with host protein profiles associated with epithelial barrier stability, although with a more pro-inflammatory profile for the Gardnerella-dominated microbiome group. Tissue samples with a highly diverse composition had a protein profile representing cell proliferation and pro-inflammatory activity.Conclusion We identified ectocervical tissue-adherent bacterial communities in all study participants of a female sex worker cohort. These communities were distinct from cervicovaginal luminal microbiota in a significant proportion of individuals. We further revealed that bacterial communities at both sites correlated with distinct host gene expression and protein levels. The tissue-adherent bacterial community could possibly act as a reservoir that seed the lumen with less optimal, non-Lactobacillus, bacteria.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Kaldhusdal, VildeKarolinska Institutet (author)
  • Czarnewski, PauloStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Natl Bioinformat Infrastruct Sweden, SciLifeLab, Solna, Sweden.(Swepub:su)pacz1456 (author)
  • Bradley, FrideborgKarolinska Institutet (author)
  • Bergström, SofiaKTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1ugb8pi (author)
  • Lajoie, JulieUniv Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada. (author)
  • Xu, JiawuMIT & Harvard, Ragon Inst MGH, Cambridge, MA USA. (author)
  • Månberg, Anna,1985-KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1g5roxd (author)
  • Kimani, JoshuaUniv Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.;Univ Nairobi, Dept Med Microbiol, Nairobi, Kenya.;Partners Hlth & Dev Afr, Nairobi, Kenya. (author)
  • Oyugi, JuliusUniv Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.;Univ Nairobi, Dept Med Microbiol, Nairobi, Kenya. (author)
  • Nilsson, PeterKTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1ws88sk (author)
  • Tjernlund, AnnelieKarolinska Institutet (author)
  • Fowke, Keith R.Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.;Univ Nairobi, Dept Med Microbiol, Nairobi, Kenya.;Partners Hlth & Dev Afr, Nairobi, Kenya.;Univ Manitoba, Dept Community Hlth Sci, Winnipeg, MB, Canada. (author)
  • Kwon, Douglas S.MIT & Harvard, Ragon Inst MGH, Cambridge, MA USA. (author)
  • Broliden, KristinaKarolinska Institutet (author)
  • Karolinska InstitutetInstitutionen för biokemi och biofysik (creator_code:org_t)

Related titles

  • In:Microbiome: Springer Nature11:12049-2618

Internet link

Find in a library

  • Microbiome (Search for host publication in LIBRIS)

To the university's database

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view