Search: WFRF:(Joshua Douglas)
> (2020-2023) >
Distinct cervical t...
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Edfeldt, GabriellaKarolinska Institutet
(author)
Distinct cervical tissue-adherent and luminal microbiome communities correlate with mucosal host gene expression and protein levels in Kenyan sex workers
- Article/chapterEnglish2023
Publisher, publication year, extent ...
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2023-03-31
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Springer Nature,2023
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-326486
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-326486URI
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https://doi.org/10.1186/s40168-023-01502-4DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-216710URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:152325704URI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20230503
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Background The majority of studies characterizing female genital tract microbiota have focused on luminal organisms, while the presence and impact of tissue-adherent ectocervical microbiota remain incompletely understood. Studies of luminal and tissue-associated bacteria in the gastrointestinal tract suggest that these communities may have distinct roles in health and disease. Here, we performed a multi-omics characterization of paired luminal and tissue samples collected from a cohort of Kenyan female sex workers.Results We identified a tissue-adherent bacterial microbiome, with a higher alpha diversity than the luminal microbiome, in which dominant genera overall included Gardnerella and Lactobacillus, followed by Prevotella, Atopobium, and Sneathia. About half of the L. iners-dominated luminal samples had a corresponding Gardnerella-dominated tissue microbiome. Broadly, the tissue-adherent microbiome was associated with fewer differentially expressed host genes than the luminal microbiome. Gene set enrichment analysis revealed that L. crispatus-dominated tissue-adherent communities were associated with protein translation and antimicrobial activity, whereas a highly diverse microbial community was associated with epithelial remodeling and pro-inflammatory pathways. Tissue-adherent communities dominated by L. iners and Gardnerella were associated with lower host transcriptional activity. Tissue-adherent microbiomes dominated by Lactobacillus and Gardnerella correlated with host protein profiles associated with epithelial barrier stability, although with a more pro-inflammatory profile for the Gardnerella-dominated microbiome group. Tissue samples with a highly diverse composition had a protein profile representing cell proliferation and pro-inflammatory activity.Conclusion We identified ectocervical tissue-adherent bacterial communities in all study participants of a female sex worker cohort. These communities were distinct from cervicovaginal luminal microbiota in a significant proportion of individuals. We further revealed that bacterial communities at both sites correlated with distinct host gene expression and protein levels. The tissue-adherent bacterial community could possibly act as a reservoir that seed the lumen with less optimal, non-Lactobacillus, bacteria.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Kaldhusdal, VildeKarolinska Institutet
(author)
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Czarnewski, PauloStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Natl Bioinformat Infrastruct Sweden, SciLifeLab, Solna, Sweden.(Swepub:su)pacz1456
(author)
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Bradley, FrideborgKarolinska Institutet
(author)
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Bergström, SofiaKTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1ugb8pi
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Lajoie, JulieUniv Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.
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Xu, JiawuMIT & Harvard, Ragon Inst MGH, Cambridge, MA USA.
(author)
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Månberg, Anna,1985-KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1g5roxd
(author)
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Kimani, JoshuaUniv Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.;Univ Nairobi, Dept Med Microbiol, Nairobi, Kenya.;Partners Hlth & Dev Afr, Nairobi, Kenya.
(author)
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Oyugi, JuliusUniv Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.;Univ Nairobi, Dept Med Microbiol, Nairobi, Kenya.
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Nilsson, PeterKTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1ws88sk
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Tjernlund, AnnelieKarolinska Institutet
(author)
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Fowke, Keith R.Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.;Univ Nairobi, Dept Med Microbiol, Nairobi, Kenya.;Partners Hlth & Dev Afr, Nairobi, Kenya.;Univ Manitoba, Dept Community Hlth Sci, Winnipeg, MB, Canada.
(author)
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Kwon, Douglas S.MIT & Harvard, Ragon Inst MGH, Cambridge, MA USA.
(author)
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Broliden, KristinaKarolinska Institutet
(author)
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Karolinska InstitutetInstitutionen för biokemi och biofysik
(creator_code:org_t)
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In:Microbiome: Springer Nature11:12049-2618
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