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Mechanism of mixed-...
Mechanism of mixed-linkage glucan biosynthesis by barley cellulose synthase–like CslF6 (1,3;1,4)-β-glucan synthase
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- Purushotham, Pallinti (författare)
- Univ Virginia, Howard Hughes Med Inst, Sch Med, Charlottesville, VA 22908 USA.;Univ Virginia, Dept Mol Physiol & Biol Phys, Sch Med, 480 Ray C Hunt Dr, Charlottesville, VA 22908 USA.
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- Ho, Ruoya (författare)
- Univ Virginia, Howard Hughes Med Inst, Sch Med, Charlottesville, VA 22908 USA.;Univ Virginia, Dept Mol Physiol & Biol Phys, Sch Med, 480 Ray C Hunt Dr, Charlottesville, VA 22908 USA.
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- Yu, Long (författare)
- Univ Adelaide, Adelaide Glyc, Waite Campus, Glen Osmond, SA 5064, Australia.;Univ Adelaide, Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia.
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- Fincher, Geoffrey B. (författare)
- Univ Adelaide, Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia.
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- Bulone, Vincent (författare)
- KTH,Glykovetenskap,Albanova VinnExcellence Center for Protein Technology, ProNova,Univ Adelaide, Adelaide Glyc, Waite Campus, Glen Osmond, SA 5064, Australia.;Univ Adelaide, Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia.;Flinders Univ S Australia, Coll Med & Publ Hlth, Hlth Sci Bldg,Bedford Pk Campus,Sturt Rd, Bedford Pk, SA 5042, Australia.
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- Zimmer, Jochen (författare)
- Univ Virginia, Howard Hughes Med Inst, Sch Med, Charlottesville, VA 22908 USA.;Univ Virginia, Dept Mol Physiol & Biol Phys, Sch Med, 480 Ray C Hunt Dr, Charlottesville, VA 22908 USA.
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Univ Virginia, Howard Hughes Med Inst, Sch Med, Charlottesville, VA 22908 USA;Univ Virginia, Dept Mol Physiol & Biol Phys, Sch Med, 480 Ray C Hunt Dr, Charlottesville, VA 22908 USA. Univ Adelaide, Adelaide Glyc, Waite Campus, Glen Osmond, SA 5064, Australia.;Univ Adelaide, Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia. (creator_code:org_t)
- American Association for the Advancement of Science (AAAS), 2022
- 2022
- Engelska.
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Ingår i: Science Advances. - : American Association for the Advancement of Science (AAAS). - 2375-2548. ; 8:45
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Mixed-linkage (1,3;1,4)-beta-glucans, which are widely distributed in cell walls of the grasses, are linear glucose polymers containing predominantly (1,4)-beta-linked glucosyl units interspersed with single (1,3)-beta-linked glucosyl units. Their distribution in cereal grains and unique structures are important determinants of dietary fibers that are beneficial to human health. We demonstrate that the barley cellulose synthase-like CslF6 enzyme is sufficient to synthesize a high-molecular weight (1,3;1,4)-beta-glucan in vitro. Biochemical and cryo-electron microscopy analyses suggest that CslF6 functions as a monomer. A conserved "switch motif" at the entrance of the enzyme's transmembrane channel is critical to generate (1,3)-linkages. There, a single-point mutation markedly reduces (1,3)-linkage formation, resulting in the synthesis of cellulosic polysaccharides. Our results suggest that CslF6 monitors the orientation of the nascent polysaccharide's second or third glucosyl unit. Register-dependent interactions with these glucosyl residues reposition the polymer's terminal glucosyl unit to form either a (1,3)- or (1,4)-beta-linkage.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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