WFRF:(Fincher Geoffrey B.)
Sökning: WFRF:(Fincher Geoffrey B.) > Mechanism of mixed-...
Mechanism of mixed-linkage glucan biosynthesis by barley cellulose synthase–like CslF6 (1,3;1,4)-β-glucan synthase
-
- Purushotham, Pallinti (författare)
- Univ Virginia, Howard Hughes Med Inst, Sch Med, Charlottesville, VA 22908 USA.;Univ Virginia, Dept Mol Physiol & Biol Phys, Sch Med, 480 Ray C Hunt Dr, Charlottesville, VA 22908 USA.
-
- Ho, Ruoya (författare)
- Univ Virginia, Howard Hughes Med Inst, Sch Med, Charlottesville, VA 22908 USA.;Univ Virginia, Dept Mol Physiol & Biol Phys, Sch Med, 480 Ray C Hunt Dr, Charlottesville, VA 22908 USA.
-
- Yu, Long (författare)
- Univ Adelaide, Adelaide Glyc, Waite Campus, Glen Osmond, SA 5064, Australia.;Univ Adelaide, Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia.
- visa fler...
- visa färre...
- Univ Virginia, Howard Hughes Med Inst, Sch Med, Charlottesville, VA 22908 USA;Univ Virginia, Dept Mol Physiol & Biol Phys, Sch Med, 480 Ray C Hunt Dr, Charlottesville, VA 22908 USA. Univ Adelaide, Adelaide Glyc, Waite Campus, Glen Osmond, SA 5064, Australia.;Univ Adelaide, Sch Agr Food & Wine, Waite Campus, Glen Osmond, SA 5064, Australia. (creator_code:org_t)
- American Association for the Advancement of Science (AAAS), 2022
- 2022
- Engelska.
- Ingår i: Science Advances. - : American Association for the Advancement of Science (AAAS). - 2375-2548. ; 8:45
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Mixed-linkage (1,3;1,4)-beta-glucans, which are widely distributed in cell walls of the grasses, are linear glucose polymers containing predominantly (1,4)-beta-linked glucosyl units interspersed with single (1,3)-beta-linked glucosyl units. Their distribution in cereal grains and unique structures are important determinants of dietary fibers that are beneficial to human health. We demonstrate that the barley cellulose synthase-like CslF6 enzyme is sufficient to synthesize a high-molecular weight (1,3;1,4)-beta-glucan in vitro. Biochemical and cryo-electron microscopy analyses suggest that CslF6 functions as a monomer. A conserved "switch motif" at the entrance of the enzyme's transmembrane channel is critical to generate (1,3)-linkages. There, a single-point mutation markedly reduces (1,3)-linkage formation, resulting in the synthesis of cellulosic polysaccharides. Our results suggest that CslF6 monitors the orientation of the nascent polysaccharide's second or third glucosyl unit. Register-dependent interactions with these glucosyl residues reposition the polymer's terminal glucosyl unit to form either a (1,3)- or (1,4)-beta-linkage.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta mer i SwePub
- Av författaren/redakt...
- Purushotham, Pal ...
- Ho, Ruoya
- Yu, Long
- Fincher, Geoffre ...
- Bulone, Vincent
- Zimmer, Jochen
- Om ämnet
-
- NATURVETENSKAP
- NATURVETENSKAP
- och Biologi
- och Biokemi och mole ...
- Artiklar i publikationen
- Science Advances
- Av lärosätet
- Kungliga Tekniska Högskolan
Sök utanför SwePub
- Sök vidare i:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - Nationella bibliotekssystem
- LIBRIS.kb.se
