Food protein-derived amyloids do not accelerate amyloid beta aggregation

QC 20230621
The deposition of proteins in the form of amyloid fibrils is closely associated with several serious diseases. The events that trigger the conversion from soluble functional proteins into insoluble amyloid are not fully understood. Many proteins that are not associated with disease can form amyloid with similar structural characteristics as the disease-associated fibrils, which highlights the potential risk of cross-seeding of disease amyloid by amyloid-like structures encountered in our surrounding. Of particular interest are common food proteins that can be transformed into amyloid under conditions similar to cooking. We here investigate cross-seeding of amyloid-beta (A beta), a peptide known to form amyloid during the development of Alzheimer's disease, by 16 types of amyloid fibrils derived from food proteins or peptides. Kinetic studies using thioflavin T fluorescence as output show that none of the investigated protein fibrils accelerates the aggregation of A beta. In at least two cases (hen egg lysozyme and oat protein isolate) we observe retardation of the aggregation, which appears to originate from interactions between the food protein seeds and A beta in aggregated form. The results support the view that food-derived amyloid is not a risk factor for development of A beta pathology and Alzheimer's disease.

Pires, Rodrigo SanchesKTH,Kemi(Swepub:kth)u1027rsu (författare)
Herneke, AnjaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences (författare)
Gowda, Vasantha,1986-KTH,Tillämpad fysikalisk kemi(Swepub:kth)u1978gco (författare)
Langton, MaudSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences(Swepub:slu)50925 (författare)
Biverstal, HenrikKarolinska Institutet (författare)
Lendel, ChristoferKTH,Kemi(Swepub:kth)u1zepbos (författare)
KTHKemi (creator_code:org_t)
Sveriges lantbruksuniversitet

Av författaren/redakt...: Rahman, M. Mahaf ...; Pires, Rodrigo S ...; Herneke, Anja; Gowda, Vasantha, ...; Langton, Maud; Biverstal, Henri ...; visa fler...; Lendel, Christof ...; visa färre...

Om ämnet

Av lärosätet: Kungliga Tekniska Högskolan; Karolinska Institutet; Sveriges Lantbruksuniversitet

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