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Rapid detection of ...
Rapid detection of viable bacteria in whole blood for early sepsis diagnosis and suseptibility testing
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- Iyengar, Sharath Narayana (author)
- KTH,Nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Dietvorst, Jiri (author)
- Instituto de Microelectrónica de Barcelona (IMB-CNM, CSIC), Universitat Autónoma de Barcelona, Cerdanyola del vallès, 08193 Barcelona, Spain, Cerdanyola del vallès
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- Ferrer-Vilanova, Amparo (author)
- Instituto de Microelectrónica de Barcelona (IMB-CNM, CSIC), Universitat Autónoma de Barcelona, Cerdanyola del vallès, 08193 Barcelona, Spain, Cerdanyola del vallès
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- Guirado, Gonzalo (author)
- Instituto de Microelectrónica de Barcelona (IMB-CNM, CSIC), Universitat Autónoma de Barcelona, Cerdanyola del vallès, 08193 Barcelona, Spain, Cerdanyola del vallès
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- Muñoz-Berbel, Xavier (author)
- Instituto de Microelectrónica de Barcelona (IMB-CNM, CSIC), Universitat Autónoma de Barcelona, Cerdanyola del vallès, 08193 Barcelona, Spain, Cerdanyola del vallès
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- Russom, Aman, Prof. 1976- (author)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi
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(creator_code:org_t)
- Chemical and Biological Microsystems Society, 2021
- 2021
- English.
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In: Proceedings MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. ; , s. 791-792
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
Close
- Sepsis is a serious medical condition characterized by a whole-body inflammatory state caused by infection. Here, we present a sepsis method for rapid detection of bacteria from whole blood in less than 5h, combining selective blood cell lysis and a sensitive colorimetric based detection method. Selective cell lysis buffer allows selective rupture of blood cells (5 min), while maintaining bacteria 100% viable. Viable bacteria metabolically reduce iron (III) complexes, initiating a photo-catalytic cascade toward Prussian Blue formation visible to the naked eye. The method is finally validated for antibiotic susceptibility testing.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- bacteria
- blood
- colorimetric
- E.coli
- Prussian blue
- Selective cell lysis
- Sepsis
Publication and Content Type
- ref (subject category)
- kon (subject category)
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