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Grass xylan structu...
Grass xylan structural variation suggests functional specialization and distinctive interaction with cellulose and lignin
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- Tryfona, Theodora (författare)
- Department of Biochemistry, School of Biological Sciences, University of Cambridge, Cambridge, CB2 1QW, UK
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- Bourdon, Matthieu (författare)
- Sainsbury Laboratory, University of Cambridge, Cambridge, CB2 1LR, UK
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- Delgado Marques, Rita (författare)
- Department of Biochemistry, School of Biological Sciences, University of Cambridge, Cambridge, CB2 1QW, UK
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- Busse-Wicher, Marta (författare)
- Department of Biochemistry, School of Biological Sciences, University of Cambridge, Cambridge, CB2 1QW, UK
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- Vilaplana, Francisco, 1979- (författare)
- KTH,Glykovetenskap
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- Stott, Katherine (författare)
- Department of Biochemistry, School of Biological Sciences, University of Cambridge, Cambridge, CB2 1QW, UK
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- Dupree, Paul (författare)
- Department of Biochemistry, School of Biological Sciences, University of Cambridge, Cambridge, CB2 1QW, UK
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(creator_code:org_t)
- 2023-01-19
- 2023
- Engelska.
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Ingår i: The Plant Journal. - : Wiley. - 0960-7412 .- 1365-313X. ; 113:5, s. 1004-1020
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Xylan is the most abundant non-cellulosic polysaccharide in grass cell walls, and it has important structural roles. The name glucuronoarabinoxylan (GAX) is used to describe this variable hemicellulose. It has a linear backbone of β-1,4-xylose (Xyl) residues that may be substituted with α-1,2-linked (4-O-methyl)-glucuronic acid (GlcA), α-1,3-linked arabinofuranose (Araf), and sometimes acetylation at the O-2 and/or O-3 positions. The role of these substitutions remains unclear, although there is increasing evidence that they affect the way xylan interacts with other cell wall components, particularly cellulose and lignin. Here, we used substitution-dependent endo-xylanase enzymes to investigate the variability of xylan substitution in grass culm cell walls. We show that there are at least three different types of xylan: (i) an arabinoxylan with evenly distributed Araf substitutions without GlcA (AXe); (ii) a glucuronoarabinoxylan with clustered GlcA modifications (GAXc); and (iii) a highly substituted glucuronoarabinoxylan (hsGAX). Immunolocalization of AXe and GAXc in Brachypodium distachyon culms revealed that these xylan types are not restricted to a few cell types but are instead widely detected in Brachypodium cell walls. We hypothesize that there are functionally specialized xylan types within the grass cell wall. The even substitutions of AXe may permit folding and binding on the surface of cellulose fibrils, whereas the more complex substitutions of the other xylans may support a role in the matrix and interaction with other cell wall components.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Trävetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Wood Science (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- arabinosylation
- Brachypodium distachyon
- cell wall molecular architecture
- glucuronidation
- grass cell wall
- grass xylan
- Miscanthus sinensis
- polysaccharide substitution patterns
- xylan–cellulose interactions
- xylan–lignin interactions
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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