Search: id:"swepub:oai:DiVA.org:kth-330492" >
Deep learning-based...
-
Jess, David UnnersjöKTH,Biofysik,Science for Life Laboratory, SciLifeLab,Univ Cologne, Fac Med, Dept Internal Med 2, Cologne, Germany.;Univ Hosp Cologne, Cologne, Germany.;Univ Cologne, Fac Med, Ctr Mol Med Cologne CMMC, Cologne, Germany.;Univ Cologne, Cologne Excellence Cluster Cellular Stress Respons, Cologne, Germany.;Karolinska Univ Hosp, MedTechLabs, Solna, Sweden.;Karolinska Inst, Dept Clin Sci Intervent & Technol, Div Renal Med, Stockholm, Sweden.
(author)
Deep learning-based segmentation and quantification of podocyte foot process morphology suggests differential patterns of foot process effacement across kidney pathologies
- Article/chapterEnglish2023
Publisher, publication year, extent ...
-
Elsevier BV,2023
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:kth-330492
-
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-330492URI
-
https://doi.org/10.1016/j.kint.2023.03.013DOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:152888065URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
QC 20230630
-
Morphological alterations at the kidney filtration barrier increase intrinsic capillary wall permeability resulting in albuminuria. However, automated, quantitative assessment of these morphological changes has not been possible with electron or light microscopy. Here we present a deep learning-based approach for segmentation and quantitative analysis of foot processes in images acquired with confocal and super-resolution fluorescence microscopy. Our method, Automatic Morphological Analysis of Podocytes (AMAP), accurately segments podocyte foot processes and quantifies their morphology. AMAP applied to a set of kidney diseases in patient biopsies and a mouse model of focal segmental glomerulosclerosis allowed for accurate and comprehensive quantification of various morphometric features. With the use of AMAP, detailed morphology of podocyte foot process effacement was found to differ between categories of kidney pathologies, showed detailed variability between diverse patients with the same clinical diagnosis, and correlated with levels of proteinuria. AMAP could potentially complement other readouts such as various omics, standard histologic/electron microscopy and blood/urine assays for future personalized diagnosis and treatment of kidney disease. Thus, our novel finding could have implications to afford an understanding of early phases of kidney disease progression and may provide supplemental information in precision diagnostics.
Subject headings and genre
-
artificial intelligence
-
kidney pathology
-
podocyte
Added entries (persons, corporate bodies, meetings, titles ...)
-
Butt, LinusUniv Cologne, Fac Med, Dept Internal Med 2, Cologne, Germany.;Univ Hosp Cologne, Cologne, Germany.;Univ Cologne, Fac Med, Ctr Mol Med Cologne CMMC, Cologne, Germany.;Univ Cologne, Cologne Excellence Cluster Cellular Stress Respons, Cologne, Germany.;Univ Hosp Cologne, Dept Internal Med 2, Kerpener Str 62, D-50937 Cologne, Germany.
(author)
-
Hoehne, MartinUniv Cologne, Fac Med, Dept Internal Med 2, Cologne, Germany.;Univ Hosp Cologne, Cologne, Germany.;Univ Cologne, Cologne Excellence Cluster Cellular Stress Respons, Cologne, Germany.
(author)
-
Sergei, GermanUniv Hosp Cologne, Cologne, Germany.;Univ Cologne, Fac Med, Ctr Mol Med Cologne CMMC, Cologne, Germany.
(author)
-
Fatehi, ArashUniv Hosp Cologne, Cologne, Germany.;Univ Cologne, Fac Med, Ctr Mol Med Cologne CMMC, Cologne, Germany.
(author)
-
Witasp, AnnaKarolinska Institutet
(author)
-
Wernerson, AnnikaKarolinska Institutet
(author)
-
Patrakka, JaakkoKarolinska Institutet
(author)
-
Hoyer, Peter F.Univ Duisburg Essen, Pediat Nephrol, Pediat 2, Essen, Germany.
(author)
-
Blom, Hans,1968-KTH,Science for Life Laboratory, SciLifeLab,Biofysik,Karolinska Univ Hosp, MedTechLabs, Solna, Sweden.(Swepub:kth)u1qhk3ta
(author)
-
Schermer, BernhardUniv Cologne, Fac Med, Dept Internal Med 2, Cologne, Germany.;Univ Hosp Cologne, Cologne, Germany.;Univ Cologne, Fac Med, Ctr Mol Med Cologne CMMC, Cologne, Germany.;Univ Cologne, Cologne Excellence Cluster Cellular Stress Respons, Cologne, Germany.
(author)
-
Bozek, KatarzynaUniv Hosp Cologne, Cologne, Germany.;Univ Cologne, Fac Med, Ctr Mol Med Cologne CMMC, Cologne, Germany.;Univ Cologne, Cologne Excellence Cluster Cellular Stress Respons, Cologne, Germany.;Ctr Mol Med Cologne CMMC, Robert Koch Str 21, D-50931 Cologne, Germany.
(author)
-
Benzing, ThomasUniv Cologne, Fac Med, Dept Internal Med 2, Cologne, Germany.;Univ Hosp Cologne, Cologne, Germany.;Univ Cologne, Fac Med, Ctr Mol Med Cologne CMMC, Cologne, Germany.;Univ Cologne, Cologne Excellence Cluster Cellular Stress Respons, Cologne, Germany.
(author)
-
KTHBiofysik
(creator_code:org_t)
Related titles
-
In:Kidney International: Elsevier BV103:6, s. 1120-11300085-25381523-1755
Internet link
Find in a library
To the university's database
- By the author/editor
-
Jess, David Unne ...
-
Butt, Linus
-
Hoehne, Martin
-
Sergei, German
-
Fatehi, Arash
-
Witasp, Anna
-
show more...
-
Wernerson, Annik ...
-
Patrakka, Jaakko
-
Hoyer, Peter F.
-
Blom, Hans, 1968 ...
-
Schermer, Bernha ...
-
Bozek, Katarzyna
-
Benzing, Thomas
-
show less...
- Articles in the publication
-
Kidney Internati ...
- By the university
-
Royal Institute of Technology
-
Karolinska Institutet