Sökning: id:"swepub:oai:DiVA.org:kth-330505" >
Identification and ...
-
Clayton-Cuch, DanielUniv Adelaide, Sch Agr Food & Wine, Adelaide Glyc, Waite Campus, Adelaide, SA 5064, Australia.;CSIRO, Waite Campus, Glen Osmond, SA 5064, Australia.
(författare)
Identification and characterisation of MdUGT78T2 as a galactosyltransferase with dual activity on flavonol and anthocyanidin substrates in red-skinned apple fruit (Malus domestica L.)
- Artikel/kapitelEngelska2023
Förlag, utgivningsår, omfång ...
-
Elsevier BV,2023
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:kth-330505
-
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-330505URI
-
https://doi.org/10.1016/j.foodchem.2023.136388DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
QC 20230630
-
Anthocyanidin and flavonol glycosides have been linked to the health-promoting effects associated with apple consumption. However, very few enzymes involved in flavonoid glycosylation have been characterised to date. Here, we present the identification and phylogenetic analysis of 234 putative glycosyltransferases involved in flavonoid biosynthesis, and detail the biochemical and structural characterisation of MdUGT78T2 as a strict galactosyltransferase involved in the formation of quercetin-3-O-galactoside and cyanidin-3-O-galactoside, the major glycoconjugates of flavonoids in apple. The enzyme is also active on other flavonoids but with a lower catalytic efficiency. Our data, complemented with gene expression analysis suggest that MdUGT78T2 synthesises the glycoconjugates at both the early and late stages of fruit development. This newly discovered type of catalytic activity can potentially be exploited for in vitro modification of flavonoids to increase their stability in food products and to modify apple fruits and other commercial crops through breeding approaches to enhance their health benefits.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
McDougal, DanielUniv Adelaide, Inst Photon & Adv Sensing IPAS, Sch Biol Sci, Adelaide, SA 5005, Australia.
(författare)
-
Schwerdt, Julian G.Univ Adelaide, Sch Agr Food & Wine, Adelaide Glyc, Waite Campus, Adelaide, SA 5064, Australia.
(författare)
-
Yu, LongUniv Adelaide, Sch Agr Food & Wine, Adelaide Glyc, Waite Campus, Adelaide, SA 5064, Australia.
(författare)
-
Shirley, NeilUniv Adelaide, Sch Agr Food & Wine, Adelaide Glyc, Waite Campus, Adelaide, SA 5064, Australia.
(författare)
-
Bradley, DavidAgilent Technol Australia Pty Ltd, Melbourne, Vic, Australia.
(författare)
-
Bruning, John B.Univ Adelaide, Inst Photon & Adv Sensing IPAS, Sch Biol Sci, Adelaide, SA 5005, Australia.
(författare)
-
Bottcher, ChristineCSIRO, Waite Campus, Glen Osmond, SA 5064, Australia.
(författare)
-
Bulone, VincentKTH,Glykovetenskap,Univ Adelaide, Sch Agr Food & Wine, Adelaide Glyc, Waite Campus, Adelaide, SA 5064, Australia.(Swepub:kth)u13o1auq
(författare)
-
Univ Adelaide, Sch Agr Food & Wine, Adelaide Glyc, Waite Campus, Adelaide, SA 5064, Australia.;CSIRO, Waite Campus, Glen Osmond, SA 5064, Australia.Univ Adelaide, Inst Photon & Adv Sensing IPAS, Sch Biol Sci, Adelaide, SA 5005, Australia.
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Food Chemistry: Elsevier BV4240308-81461873-7072
Internetlänk
Hitta via bibliotek
Till lärosätets databas