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  • Englund, EliasKTH,Science for Life Laboratory, SciLifeLab,Skolan för kemi, bioteknologi och hälsa (CBH),Joint BioEnergy Institute, Emeryville, CA, USA (författare)

Biosensor Guided Polyketide Synthases Engineering for Optimization of Domain Exchange Boundaries

  • Artikel/kapitelEngelska2023

Förlag, utgivningsår, omfång ...

  • Springer Nature,2023
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:kth-334951
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-334951URI
  • https://doi.org/10.1038/s41467-023-40464-xDOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

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Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • QC 20230830
  • Type I modular polyketide synthases (PKSs) are multi-domain enzymes functioning like assembly lines. Many engineering attempts have been made for the last three decades to replace, delete and insert new functional domains into PKSs to produce novel molecules. However, inserting heterologous domains often destabilize PKSs, causing loss of activity and protein misfolding. To address this challenge, here we develop a fluorescence-based solubility biosensor that can quickly identify engineered PKSs variants with minimal structural disruptions. Using this biosensor, we screen a library of acyltransferase (AT)-exchanged PKS hybrids with randomly assigned domain boundaries, and we identify variants that maintain wild type production levels. We then probe each position in the AT linker region to determine how domain boundaries influence structural integrity and identify a set of optimized domain boundaries. Overall, we have successfully developed an experimentally validated, high-throughput method for making hybrid PKSs that produce novel molecules.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Schmidt, MatthiasJoint BioEnergy Institute, Emeryville, CA, USA; Institute of Applied Microbiology, Aachen Biology and Biotechnology (ABBt), RWTH Aachen University, Aachen, Germany; Biological Systems and Engineering Division, Lawrence Berkeley National laboratory, Berkeley, CA, USA (författare)
  • Nava, Alberto A.Joint BioEnergy Institute, Emeryville, CA, USA; Department of Chemical & Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA (författare)
  • Klass, SarahJoint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National laboratory, Berkeley, CA, USA; Department of Chemical & Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA (författare)
  • Keiser, LeahJoint BioEnergy Institute, Emeryville, CA, USA; Department of Chemical & Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA (författare)
  • Dan, QingyunJoint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National laboratory, Berkeley, CA, USA (författare)
  • Katz, LeonardJoint BioEnergy Institute, Emeryville, CA, USA; QB3, University of California, Berkeley, Berkeley, CA, USA (författare)
  • Yuzawa, SatoshiJoint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National laboratory, Berkeley, CA, USA; Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan; Graduate school of Media and Governance, Keio University, Fujisawa, Kanagawa, Japan (författare)
  • Keasling, Jay D.Joint BioEnergy Institute, Emeryville, CA, USA; Biological Systems and Engineering Division, Lawrence Berkeley National laboratory, Berkeley, CA, USA; Department of Chemical & Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA; QB3, University of California, Berkeley, Berkeley, CA, USA; Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA; Center for Biosustainability, Danish Technical University, Lyngby, Denmark; Center for Synthetic biochemistry, Institute for Synthetic biology, Shenzhen Institute of Advanced Technology, Shenzhen, China (författare)
  • KTHScience for Life Laboratory, SciLifeLab (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Nature Communications: Springer Nature14:12041-1723

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