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Metabolite interact...
Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation
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- Sporre, Emil (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Systembiologi
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- Karlsen, Jan (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
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- Schriever, Karen (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Ytbehandlingsteknik
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- Asplund-Samuelsson, Johannes, 1987- (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
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- Janasch, Markus (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Systembiologi,Department of Biotechnology and Nanomedicine, SINTEF Industry, 7465, Trondheim, Norway
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- Strandberg, Linnéa (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
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- Karlsson, Anna (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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- Kotol, David (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
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- Zeckey, Luise (författare)
- KTH,Systembiologi,Science for Life Laboratory, SciLifeLab
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- Piazza, Ilaria (författare)
- Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
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- Syrén, Per-Olof (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Ytbehandlingsteknik
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- Edfors, Fredrik (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Systembiologi
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- Hudson, Elton P. (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Systembiologi
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(creator_code:org_t)
- Springer Nature, 2023
- 2023
- Engelska.
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Ingår i: Communications Biology. - : Springer Nature. - 2399-3642. ; 6:1
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Metabolite-level regulation of enzyme activity is important for microbes to cope with environmental shifts. Knowledge of such regulations can also guide strain engineering for biotechnology. Here we apply limited proteolysis-small molecule mapping (LiP-SMap) to identify and compare metabolite-protein interactions in the proteomes of two cyanobacteria and two lithoautotrophic bacteria that fix CO2 using the Calvin cycle. Clustering analysis of the hundreds of detected interactions shows that some metabolites interact in a species-specific manner. We estimate that approximately 35% of interacting metabolites affect enzyme activity in vitro, and the effect is often minor. Using LiP-SMap data as a guide, we find that the Calvin cycle intermediate glyceraldehyde-3-phosphate enhances activity of fructose-1,6/sedoheptulose-1,7-bisphosphatase (F/SBPase) from Synechocystis sp. PCC 6803 and Cupriavidus necator in reducing conditions, suggesting a convergent feed-forward activation of the cycle. In oxidizing conditions, glyceraldehyde-3-phosphate inhibits Synechocystis F/SBPase by promoting enzyme aggregation. In contrast, the glycolytic intermediate glucose-6-phosphate activates F/SBPase from Cupriavidus necator but not F/SBPase from Synechocystis. Thus, metabolite-level regulation of the Calvin cycle is more prevalent than previously appreciated.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
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Sporre, Emil
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Karlsen, Jan
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Schriever, Karen
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Asplund-Samuelss ...
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Janasch, Markus
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Strandberg, Linn ...
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visa fler...
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Karlsson, Anna
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Kotol, David
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Zeckey, Luise
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Piazza, Ilaria
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Syrén, Per-Olof
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Edfors, Fredrik
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Hudson, Elton P.
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NATURVETENSKAP
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och Bioinformatik oc ...
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NATURVETENSKAP
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Kungliga Tekniska Högskolan