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- Giang, Kim AnhKTH,Proteinvetenskap (author)
Construction of Synthetic Antibody Phage Display Libraries
- Article/chapterEnglish2023
Publisher, publication year, extent ...
- Springer Nature,2023
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:kth-337791
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-337791URI
- https://doi.org/10.1007/978-1-0716-3381-6_4DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
Classification
Notes
- QC 20231009
- Synthetic antibody libraries provide a vast resource of renewable antibody reagents that can rival natural antibodies and be rapidly isolated through controlled in vitro selections. Use of highly optimized human frameworks enables the incorporation of defined diversity at positions that are most likely to contribute to antigen recognition. This protocol describes the construction of synthetic antibody libraries based on a single engineered human autonomous variable heavy domain scaffold with diversity in all three complementarity-determining regions. The resulting libraries can be used to generate recombinant domain antibodies targeting a wide range of protein antigens using phage display. Furthermore, analogous methods can be used to construct antibody libraries based on larger antibody fragments or second-generation libraries aimed to fine-tune antibody characteristics including affinity, specificity, and manufacturability. The procedures rely on standard reagents and equipment available in most molecular biology laboratories.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Immunologi inom det medicinska området hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Immunology in the medical area hsv//eng
- Antibody fragment
- Degenerate oligonucleotide
- Domain antibody
- Human antibody
- Phage display
- Protein engineering
Added entries (persons, corporate bodies, meetings, titles ...)
- Sidhu, Sachdev S.School of Pharmacy, University of Waterloo, Kitchener, ON, Canada (author)
- Nilvebrant, Johan,1982-KTH,Proteinteknologi(Swepub:kth)u17j1dcn (author)
- KTHProteinvetenskap (creator_code:org_t)
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- In:Methods in Molecular Biology: Springer Nature2702, s. 59-751064-37451940-6029
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