Sökning: WFRF:(Zhang Tianyi) >
MiR-378a suppresses...
MiR-378a suppresses tenogenic differentiation and tendon repair by targeting at TGF-β2
-
- Liu, Yang (författare)
- Lund University,Lunds universitet,Avdelningen för molekylärmedicin och genterapi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Molecular Medicine and Gene Therapy,Department of Laboratory Medicine,Faculty of Medicine,Chinese University of Hong Kong
-
- Feng, Lu (författare)
- Chinese University of Hong Kong
-
- Xu, Jia (författare)
- Shanghai Jiao Tong University
-
visa fler...
-
- Yang, Zhengmeng (författare)
- Chinese University of Hong Kong
-
- Wu, Tianyi (författare)
- Shanghai Jiao Tong University
-
- Zhang, Jiajun (författare)
- Chinese University of Hong Kong
-
- Shi, Liu (författare)
- Chinese University of Hong Kong
-
- Zhu, Dahai (författare)
- Peking Union Medical College
-
- Zhang, Jinfang (författare)
- Guangzhou University of Chinese Medicine
-
- Li, Gang (författare)
- Chinese University of Hong Kong
-
visa färre...
-
(creator_code:org_t)
- 2019-03-29
- 2019
- Engelska.
-
Ingår i: Stem Cell Research and Therapy. - : Springer Science and Business Media LLC. - 1757-6512. ; 10:1
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
https://stemcellres....
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: Tendons are a crucial component of the musculoskeletal system and responsible for transmission forces derived from muscle to bone. Patients with tendon injuries are often observed with decreased collagen production and matrix degeneration, and healing of tendon injuries remains a challenge as a result of limited understanding of tendon biology. Recent studies highlight the contribution of miR-378a on the regulation gene expression during tendon differentiation. Methods: We examined the tendon microstructure and tendon repair with using miR-378a knock-in transgenic mice, and the tendon-derived stem cells were also isolated from transgenic mice to study their tenogenic differentiation ability. Meanwhile, the expression levels of tenogenic markers were also examined in mouse tendon-derived stem cells transfected with miR-378a mimics during tenogenic differentiation. With using online prediction software and luciferase reporter assay, the binding target of miR-378a was also studied. Results: Our results indicated miR-378a impairs tenogenic differentiation and tendon repair by inhibition collagen and extracellular matrix production both in vitro and in vivo. We also demonstrated that miR-378a exert its inhibitory role during tenogenic differentiation through binding at TGFβ2 by luciferase reporter assay and western blot. Conclusions: Our investigation suggests that miR-378a could be considered as a new potential biomarker for tendon injury diagnosis or drug target for a possible therapeutic approach in future clinical practice.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Liu, Yang
-
Feng, Lu
-
Xu, Jia
-
Yang, Zhengmeng
-
Wu, Tianyi
-
Zhang, Jiajun
-
visa fler...
-
Shi, Liu
-
Zhu, Dahai
-
Zhang, Jinfang
-
Li, Gang
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Cell och molekyl ...
- Artiklar i publikationen
-
Stem Cell Resear ...
- Av lärosätet
-
Lunds universitet