Antibody-loading of biological nanocarrier vesicles derived from red-blood-cell membranes

• Antibodies, disruptive potent therapeutic agents against pharmacological targets, face a barrier crossing immune-system and cellular-membranes. To overcome these, various strategies have been explored including shuttling via liposomes or bio-camouflaged nanoparticles.Here, we demonstrate the feasibility to load antibodies into exosome-mimetic nanovesicles derived from human red-blood-cell-membranes. The goat-anti-chicken antibodies are loaded into erythrocyte-membrane derived nanovesicles and their loading yields are characterized and compared with smaller dUTP-cargo. Applying dual-color coincident fluorescence burst methodology, the loading yield of nanocarriers is profiled at single-vesicle level overcoming their size-heterogeneity and achieving a maximum of 38-41% antibody-loading yield at peak radius of 52 nm. The average of 14 % yield and more than two antibodies per vesicle is estimated, comparable to those of dUTP-loaded nanovesicles after additional purification through exosomespin-column. These results suggest a promising route for enhancing biodistribution andintracellular accessibility for therapeutic antibodies using novel, biocompatible, and lowimmunogenicity nanocarriers, suitable for large-scale pharmacological applications.

Subject headings

TEKNIK OCH TEKNOLOGIER  -- Nanoteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Nano-technology (hsv//eng)

Keyword

Antibody loading

nanovesicles

biomimetic drug carriers

fluorescence correlation spectroscopy

coincident fluorescence burst analysis

atomic force microscopy

Optics and Photonics

Optik och fotonik

Biological and Biomedical Physics

Biologisk och biomedicinsk fysik

Publication and Content Type

vet (subject category)

ovr (subject category)