Sequential sequencing by synthesis and the next-generation sequencing revolution

Uhlén, MathiasKTH,Science for Life Laboratory, SciLifeLab,Systembiologi,Karolinska Inst, Dept Neurosci, Stockholm, Sweden. (författare)

QC 20231222
The impact of next-generation sequencing (NGS) cannot be overestimated. The technology has transformed the field of life science, contributing to a dramatic expansion in our understanding of human health and disease and our understanding of biology and ecology. The vast majority of the major NGS systems today are based on the concept of 'sequencing by synthesis' (SBS) with sequential detection of nucleotide incorporation using an engineered DNA polymerase. Based on this strategy, various alternative platforms have been developed, including the use of either native nucleotides or reversible terminators and different strategies for the attachment of DNA to a solid support. In this review, some of the key concepts leading to this remarkable development are discussed.

Quake, Stephen R.Stanford Univ, Dept Bioengn, Stanford, CA USA.;Stanford Univ, Dept Appl Phys, Stanford, CA USA.;Chan Zuckerberg Initiat, Redwood City, CA USA.;Chan Zuckerberg Initiat, Stanford, CA USA. (författare)
KTHScience for Life Laboratory, SciLifeLab (creator_code:org_t)

Ingår i:Trends in Biotechnology: Elsevier BV41:12, s. 1565-15720167-77991879-3096

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