Biochemical and in silico characterization of glycosyltransferases from red sweet cherry (Prunus avium L.) reveals their broad specificity toward phenolic substrates

Clayton-Cuch, DanielAdelaide Glycomics, University of Adelaide, School of Agriculture, Food and Wine, Waite Campus, Adelaide, South Australia, Australia, Waite Campus; CSIRO, Waite Campus, Glen Osmond, SA 5064, Australia, Waite Campus (författare)

QC 20240301
Polyphenolic compounds are a class of phytonutrients that play important roles in plants and contribute to human health when incorporated into our diet through fruit consumption. A large proportion occur as glycoconjugates but the enzymes responsible for their glycosylation are poorly characterized. Here, we report the biochemical and structural characterization of two glycosyltransferases from sweet cherry named PaUGT1 and PaUGT2. Both are promiscuous glucosyltransferases active on diverse anthocyanidins and flavonols, as well as phenolic acids in the case of PaUGT1. They also exhibit weaker galactosyltransferase activity. The expression of the gene encoding PaUGT1, the most active of the two proteins, follows anthocyanin accumulation during fruit ripening, suggesting that this enzyme is the primary glycosyltransferase involved in flavonoid glycosylation in sweet cherry. It can potentially be used to synthesize diverse glycoconjugates of flavonoids for integration into bioactive formulations, and for generating new fruit cultivars with enhanced health-promoting properties using breeding methods.

Yu, LongAdelaide Glycomics, University of Adelaide, School of Agriculture, Food and Wine, Waite Campus, Adelaide, South Australia, Australia, Waite Campus (författare)
McDougal, DanielInstitute for Photonics and Advanced Sensing, (IPAS), School of Biological Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia (författare)
Burbidge, Crista A.CSIRO, Waite Campus, Glen Osmond, SA 5064, Australia, Waite Campus (författare)
Bruning, John B.Institute for Photonics and Advanced Sensing, (IPAS), School of Biological Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia (författare)
Bradley, DavidAgilent Technologies Australia Pty Ltd, Mulgrave, Melbourne, Victoria, Australia, Mulgrave (författare)
Böttcher, ChristineCSIRO, Waite Campus, Glen Osmond, SA 5064, Australia, Waite Campus (författare)
Bulone, VincentKTH,Glykovetenskap,Adelaide Glycomics, University of Adelaide, School of Agriculture, Food and Wine, Waite Campus, Adelaide, South Australia, Australia, Waite Campus(Swepub:kth)u13o1auq (författare)
Adelaide Glycomics, University of Adelaide, School of Agriculture, Food and Wine, Waite Campus, Adelaide, South Australia, Australia, Waite Campus; CSIRO, Waite Campus, Glen Osmond, SA 5064, Australia, Waite CampusAdelaide Glycomics, University of Adelaide, School of Agriculture, Food and Wine, Waite Campus, Adelaide, South Australia, Australia, Waite Campus (creator_code:org_t)

Av författaren/redakt...: Clayton-Cuch, Da ...; Yu, Long; McDougal, Daniel; Burbidge, Crista ...; Bruning, John B.; Bradley, David; visa fler...; Böttcher, Christ ...; Bulone, Vincent; visa färre...

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