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Mechano-regulation ...
Mechano-regulation by clathrin pit-formation and passive cholesterol-dependent tubules during de-adhesion
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- Mandal, Tithi (author)
- Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India
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- Biswas, Arikta (author)
- Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India; Mechanobiology Institute, National University of Singapore, 5A Engineering Drive 1, Singapore, 117411, Singapore
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- Ghosh, Tanmoy (author)
- Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India
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- Manikandan, Sreekanth (author)
- Stockholms universitet,Nordiska institutet för teoretisk fysik (Nordita),Fysikum,Nordita SU
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- Kundu, Avijit (author)
- Department of Physical Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India; Experimental Physics I, Universität Bayreuth, Universitätsstraße 30, 95447, Bayreuth, Germany
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- Banerjee, Ayan (author)
- Department of Physical Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India
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- Mitra, Dhrubaditya (author)
- Stockholms universitet,KTH,Nordic Institute for Theoretical Physics NORDITA,Nordiska institutet för teoretisk fysik (Nordita)
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- Sinha, Bidisha (author)
- Department of Biological Sciences, Indian Institute of Science Education and Research Kolkata, Nadia, Mohanpur, 741246, India
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(creator_code:org_t)
- Springer Nature, 2024
- 2024
- English.
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In: Cellular and Molecular Life Sciences (CMLS). - : Springer Nature. - 1420-682X .- 1420-9071. ; 81:1
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Adherent cells ensure membrane homeostasis during de-adhesion by various mechanisms, including endocytosis. Although mechano-chemical feedbacks involved in this process have been studied, the step-by-step build-up and resolution of the mechanical changes by endocytosis are poorly understood. To investigate this, we studied the de-adhesion of HeLa cells using a combination of interference reflection microscopy, optical trapping and fluorescence experiments. We found that de-adhesion enhanced membrane height fluctuations of the basal membrane in the presence of an intact cortex. A reduction in the tether force was also noted at the apical side. However, membrane fluctuations reveal phases of an initial drop in effective tension followed by saturation. The area fractions of early (Rab5-labelled) and recycling (Rab4-labelled) endosomes, as well as transferrin-labelled pits close to the basal plasma membrane, also transiently increased. On blocking dynamin-dependent scission of endocytic pits, the regulation of fluctuations was not blocked, but knocking down AP2-dependent pit formation stopped the tension recovery. Interestingly, the regulation could not be suppressed by ATP or cholesterol depletion individually but was arrested by depleting both. The data strongly supports Clathrin and AP2-dependent pit-formation to be central to the reduction in fluctuations confirmed by super-resolution microscopy. Furthermore, we propose that cholesterol-dependent pits spontaneously regulate tension under ATP-depleted conditions.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Excess area regulation
- Membrane homeostasis
- Tension propagation
Publication and Content Type
- ref (subject category)
- art (subject category)
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