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Identification of M...
Identification of Major Immune Cell Lineages with DBS-Pro
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- Höjer, Pontus (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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- Nagy, Abel (författare)
- KTH,Proteinvetenskap
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- Siga, Humam (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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visa fler...
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- Wang, Jun (författare)
- Karolinska Institutet, Department of Women's and Children's Health, Biomedicum, Solna, Sweden
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- Jönsson, Håkan, PhD, 1979- (författare)
- KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap,Nanobioteknologi
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- Brodin, Petter (författare)
- Karolinska Institutet, Department of Women's and Children's Health, Biomedicum, Solna, Sweden; Imperial College London, Department of immunology and Inflammation, London, UK
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- Eriksson Karlström, Amelie (författare)
- KTH,Albanova VinnExcellence Center for Protein Technology, ProNova,Proteinvetenskap,Proteinteknologi
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- Ahmadian, Afshin (författare)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Proteins play a pivotal role in cellular function and heterogeneity. Understanding cellular diversity at the proteome level necessitates sensitive single-cell assays with high throughput. While current sequencing-based methods offer promise, they often face limitations, including reliance on expensive and inaccessible commercial platforms. Here, we have adopted the DBS-Pro method, utilizing site-specific oligonucleotide-conjugated antibodies, to analyze surface proteins in single cells. The method uses cheap degenerated barcode oligonucleotides and a simple microfluidics setup for cell encapsulation. A sample of PBMCs was examined using a panel targeting six separate immune cell markers. Using this panel we could quantify marker expression on 1,307 cells, identifying major immune cell lineages including CD4+ T-cells, CD8+ T-cells, monocytes, and B-cells. While recognizing the need for protocol improvements, our results present a promising approach for single-cell proteomics.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Publikations- och innehållstyp
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