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- Parks, LukeKTH,Proteinvetenskap (author)
Directed evolution of an affibody molecule for carbonic anhydrase IX using E. coli display and fluorescence-activated cell sorting
- BookEnglish
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- LIBRIS-ID:oai:DiVA.org:kth-346203
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-346203URI
Supplementary language notes
- Language:English
- Summary in:English
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Notes
- QC20240507
- Carbonic anhydrase IX (CAIX) is a transmembrane enzyme that plays an important role in pH regulation and cellular homeostasis. Overexpression of CAIX is commonly observed in various solid tumors, making it an attractive target for cancer therapy. Affibody molecules have previously been developed for CAIX and demonstrated potential as tracers for molecular imaging in murine xenografted tumor models. Here, we further develop such affibody molecules by displaying a designed affibody library on the surface of Escherichia coli, followed by isolation of CAIX-binding variants by a combination of magnetic-assisted cell sorting and fluorescence-activated cell sorting. The sortings were successful and screening of candidates after selection revealed several variants with improved properties, most notably an increase in affinity and CAIX cell binding at 37°C and 42°C.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- Affibody molecule
- carbonic anhydrase IX
- Escherichia coli surface display
- MACS
- FACS
- directed evolution
- Biotechnology
- Bioteknologi
Added entries (persons, corporate bodies, meetings, titles ...)
- Klint, SusanneAffibody Medical AB, Stockholm, Sweden (author)
- Gunneriusson, ElinAffibody Medical AB, Stockholm, Sweden (author)
- Frejd, FredrikAffibody Medical AB, Stockholm, Sweden (author)
- Ståhl, Stefan,1961-KTH,Albanova VinnExcellence Center for Protein Technology, ProNova,Proteinteknologi(Swepub:kth)u1svy8i4 (author)
- Löfblom, JohnKTH,Skolan för teknikvetenskap (SCI),Proteinvetenskap,Proteinteknologi(Swepub:kth)u1z7cgkl (author)
- KTHProteinvetenskap (creator_code:org_t)
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- Royal Institute of Technology
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