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A modular click app...
A modular click approach to glycosylated polymeric beads : Design, synthesis and preliminary lectin, recognition studies
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Chen, Gaojian (författare)
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Tao, Lei (författare)
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Mantovani, Giuseppe (författare)
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Geng, J. (författare)
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- Nyström, Daniel (författare)
- KTH,Fiber- och polymerteknologi
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Haddleton, David M. (författare)
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(creator_code:org_t)
- 2007-09-15
- 2007
- Engelska.
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Ingår i: Macromolecules. - : American Chemical Society (ACS). - 0024-9297 .- 1520-5835. ; 40:21, s. 7513-7520
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Covalent immobilization of a range of carbohydrate derivatives onto polymeric resin beads is described. Copper-catalyzed Huisgen [2 + 3] cycloaddition (often termed click chemistry) was used to graft mannose-containing azides to complementarily functionalized alkyne surfaces, namely (a) Wang resin or (b) Rasta particles consisting of a clickable alkyne polymer loose outer shell and a Wang resin inner core. For the second approach, Wang resin beads were first converted into immobilized living radical polymerization initiators with subsequent polymerization of trimethylsilanyl-protected propargyl methacrylate followed by deprotection with TBAF to yield the desired polyalkyne clickable scaffold. The appropriate (x-mannopyranoside azide was then clicked onto the bead to give a mannose functionalized Rasta resin. IR, gel-phase H-1 NMR, and elemental analysis have been used to characterize the modified resins. The binding abilities of these D-mannose-modified particles were subsequently tested using fluorescein-labeled Concanavalin A (Con A), a lectin that binds certain mannose-containing molecules. Preliminary results indicated that the novel glyco-hybrid materials presented in this work are able to efficiently recognize mannose-binding model lectins such as Con A, opening the way for their potential application in affinity chromatography, sensors, and other protein recognition/separation fields.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- LIVING RADICAL POLYMERIZATION
- PROTEIN-CARBOHYDRATE INTERACTIONS
- HUISGEN CYCLOADDITION PROCESS
- CONCANAVALIN-A
- GRAFT-POLYMERIZATION
- STATIONARY PHASES
- BLOCK-COPOLYMERS
- MEMBRANE-SURFACE
- TERMINAL ALKYNES
- SCAVENGER RESIN
- Chemistry
- Kemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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