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Exploring the use of soft X-ray microscopy for imaging subcellular structures of the inner ear

Johansson, Göran A. (author)
KTH,Fysik
Khanna, S M (author)
Nair, A (author)
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Mannstrom, P (author)
Karolinska Institutet
Denbeaux, G (author)
Ulfendahl, M (author)
Karolinska Institutet
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 (creator_code:org_t)
Wiley, 2004
2004
English.
In: Journal of Microscopy. - : Wiley. - 0022-2720 .- 1365-2818. ; 215, s. 203-212
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • The soft X-ray microscope at the Lawrence Berkeley National Laboratory was developed for visualization of biological tissue. Soft X-ray microscopy provides high-resolution visualization of hydrated, non-embedded and non-sectioned cells and is thus potentially an alternative to transmission electron microscopy. Here we show for the first time soft X-ray micrographs of structures isolated from the guinea-pig inner ear. Sensory outer hair cells and supporting pillar cells are readily visualized. In the hair cells, individual stereocilia can easily be identified within the apical hair bundle. The underlying cuticular plate is, however, too densely composed or too thick to be clearly visualized, and thus appears very dark. The cytoplasmic structures protruding from the cuticular plates as well as the fibrillar material surrounding and projecting from the cell nuclei can be seen. In the pillar cells the images reveal individual microtubule bundles. Soft X-ray images of the acellular tectorial membrane and thin two-layered Reissner's membrane display a level of resolution comparable to low-power electron microscopy.

Subject headings

NATURVETENSKAP  -- Fysik (hsv//swe)
NATURAL SCIENCES  -- Physical Sciences (hsv//eng)

Keyword

hair cell
inner ear
Reissner's membrane
soft X-ray microscopy
tectorial membrane

Publication and Content Type

ref (subject category)
art (subject category)

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