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Selection of anion exchangers for detoxification of dilute-acid hydrolysates from spruce

Horvath, I. S. (författare)
Sjöde, A. (författare)
Nilvebrant, N. O. (författare)
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Zagorodni, Andrei (författare)
KTH,Materialvetenskap
Jönsson, L. J. (författare)
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 (creator_code:org_t)
2004
2004
Engelska.
Ingår i: Applied Biochemistry and Biotechnology. - 0273-2289 .- 1559-0291. ; 113, s. 525-538
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Six anion-exchange resins with different properties were compared with respect to detoxification of a dilute-acid hydrolysate of spruce prior to ethanolic fermentation with Saccharomyces cerevisiae. The six resins encompassed strong and weak functional groups as well as styrene-, phenol-, and acrylic-based matrices. In an analytical experimental series, fractions from columns packed with the different resins were analyzed regarding pH, glucose, furfural, hydroxymethylfurfural, phenolic compounds, levulinic acid, acetic acid, formic acid, and sulfate. An initial adsorption of glucose occurred in the strong alkaline environment and led to glucose accumulation at a later stage. Acetic and levulinic acid passed through the column before formic acid, whereas sulfate had the strongest affinity. In a preparative experimental series, one fraction from each of six columns packed with the different resins was collected for assay of the fermentability and analysis of glucose, mannose, and fermentation inhibitors. The fractions collected from strong anion-exchange resins with styrene-based matrices displayed the best fermentability: a sevenfold enhancement of ethanol productivity compared with untreated hydrolysate. Fractions from a strong anion exchanger with acrylic-based matrix and a weak exchanger with phenol-based resin displayed an intermediate improvement in fermentability, a four- to fivefold increase in ethanol productivity. The fractions from two weak exchangers with styrene- and acrylic-based matrices displayed a twofold increase in ethanol productivity. Phenolic compounds were more efficiently removed by resins with styrene- and phenol-based matrices than by resins with acrylic-based matrices.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Saccharomyces cerevisiae
anion-exchange resins
hydrolysate
fermentability

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ref (ämneskategori)
art (ämneskategori)

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