Tuning of dissolved oxygen and pH PID control parameters in large scale bioreactor by lag control

QC 20120507
A method has been developed to tune the DO and pH controller PID parameters for pilot / large scale mammalian cultivation. Our approach is to identify a model of the variable to be controlled (e.g. DO, pH) and to design several possible PID controllers based on this model. The controllers were first tested in computer simulations, followed by wet simulation and finally the best controller was tested on the real process. The approach is developed for the tuning of the DO controller of a 50 L bioreactor using microbubble continuous oxygen flow. The method, called lag control here, is based on a lead lag control design using Bode analysis where the prediction part is omitted. Experiments show that the approach results in a highly satisfactory DO control. The oxygen microbubbles were almost completely consumed before reaching the liquid surface so the oxygen flow used to maintain the DO gave an excellent indication of the cellular oxygen consumption. The control system was robust against all the perturbations, i.e. cell growth, cell bleed, addition of air-saturated fresh medium, DO set point change and a second gas sparger used to strip out the carbon dioxide. This approach was also successfully used for the tuning of a 400 L bioreactor DO controller and pH controller.

Hjalmarsson, Håkan,1962-KTH,Reglerteknik,ACCESS Linnaeus Centre,System Identification Group(Swepub:kth)u10a8l40 (författare)
KTHBioprocessteknik (creator_code:org_t)

Ingår i:Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT)Dordrecht : Springer Netherlands, s. 327-3309789400708839

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