Altering the specificity of subtilisin B. lentus by combining site-directed mutagenesis and chemical modification

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: WFRF:(Berglund M.) > (1995-1999) > Altering the specif...

Details
MARC

Berglund, P.University of Toronto, Canada (author)

Altering the specificity of subtilisin B. lentus by combining site-directed mutagenesis and chemical modification

Article/chapterEnglish1996

Publisher, publication year, extent ...

1996
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:kth-74867
https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-74867URI
https://doi.org/10.1016/0960-894X(96)00467-2DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

References: Cornish, V.W., Mendel, D., Schultz, P.G., (1995) Angew. Chem., Int. Ed. Engl., 34, p. 621; Polgar, L., Bender, M.L., (1966) J. Am. Chem. Soc., 88, p. 3153; Neet, K.E., Koshland D.E., Jr., (1966) Proc. Natl. Acad. Sci. USA, 56, p. 1606; Hilvert, D., Kaiser, E.T., (1985) J. Am. Chem. Soc., 107, p. 5805; Radziejewski, C., Ballou, D.P., Kaiser, E.T., (1985) J. Am. Chem. Soc., 107, p. 3352; Stewart, K.D., Radziejewski, C., Kaiser, E.T., (1986) J. Am. Chem. Soc., 108, p. 3480; Rokita, S.E., Kaiser, E.T., (1986) J. Am. Chem. Soc., 108, p. 4984; Kokubo, T., Sassa, S., Kaiser, E.T., (1987) J. Am. Chem. Soc., 109, p. 606; Kaiser, E.T., (1988) Angew. Chem., Int. Ed. Engl., 27, p. 913; Hilvert, D., Hatanaka, Y., Kaiser, E.T., (1988) J. Am. Chem. Soc., 110, p. 682; Wu, Z.-P., Hilvert, D., (1989) J. Am. Chem. Soc., 111, p. 4514; Bell, I.M., Fisher, M.L., Wu, Z.-P., Hilvert, D., (1993) Biochemistry, 32, p. 3754; Peterson, E.B., Hilvert, D., (1995) Biochemistry, 34, p. 6616; Suckling, C.J., Zhu, L.-M., (1993) Bioorg. Med. Chem. Lett., 3, p. 531; Smith, D.J., Kenyon, G.L., (1974) J. Biol. Chem., 249, p. 3317; Kenyon, G.L., Bruice, T.W., (1977) Methods Enzymol., 47, p. 407; Brocklehurst, K., (1979) Int. J. Biochem, 10, p. 259; Bruice, T.W., Kenyon, G.L., (1982) J. Protein Chem., 1, p. 47; Wynn, R., Richards, F.M., (1995) Methods Enzymol., 257, p. 351; Bech, L.M., Breddam, K., (1988) Carlsberg Res. Commun., 53, p. 381; Wynn, R., Harkins, P.C., Richards, F.M., Fox, R.O., (1996) Protein Sci., 5, p. 1026; GrÃžn, H., Bech, L.M., Branner, S., Breddam, K., (1990) Eur. J. Biochemistry, 194, p. 897; noteStabile, M.R., Lai, W.G., DeSantis, G., Gold, M., Jones, J.B., Mitchinson, C., Bott, R.R., Liu, C.-C., (1996) Bioorg. Med. Chem. Lett., , in press; noteCleland, W.W., (1964) Biochemistry, 3, p. 480; Graycar, T.P., Bott, R.R., Caldwell, R.M., Dauberman, J.L., Lad, P.J., Power, S.D., Sagar, I.H., Estell, D.A., (1992) Ann. N. Y. Acad. Sci., 672, p. 71; Hsia, C.Y., Ganshaw, G., Paech, C., Murray, C.J., (1996) Anal. Biochem., , in press; Waley, S.G., (1982) Biochem. J., 205, p. 631 NR 20140805
The thiol side chain of the M222C mutant of the subtilisin from Bacillus lentus (SBL) has been chemically modified by methyl-, aminoethyl-, and sulfonatoethylthiosulfonate reagents. Introduction of charged residues into the active site of the enzyme reduced the catalytic efficiency with Suc-AAPF-pNA as the substrate, but resulted in better binding of sterically demanding boronic acid inhibitors.

Subject headings and genre

TEKNIK OCH TEKNOLOGIER Industriell bioteknik Biokatalys och enzymteknik hsv//swe
ENGINEERING AND TECHNOLOGY Industrial Biotechnology Biocatalysis and Enzyme Technology hsv//eng
boronic acid derivative
subtilisin
article
Eggerthella lenta
enzyme activity
enzyme modification
site directed mutagenesis
Bacillus lentus

Added entries (persons, corporate bodies, meetings, titles ...)

Stabile, M. R. (author)
Gold, M. (author)
Jones, J. B. (author)
Mitchinson, C. (author)
Bott, R. R. (author)
Graycar, T. P. (author)
University of Toronto, Canada (creator_code:org_t)

Related titles

In:Bioorganic & Medicinal Chemistry Letters6:21, s. 2507-25120960-894X1464-3405

Internet link

Find in a library

Bioorganic & Medicinal Chemistry Letters (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: Berglund, P.; Stabile, M. R.; Gold, M.; Jones, J. B.; Mitchinson, C.; Bott, R. R.; show more...; Graycar, T. P.; show less...

About the subject

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Biocatalysis and ...

Articles in the publication: Bioorganic & Med ...

By the university: Royal Institute of Technology

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se