The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II

• CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

electron microscopy; transcription; protein structure; yeast; Schizosaccharomyces pombe

NATURAL SCIENCES

NATURVETENSKAP

NATURAL SCIENCES

NATURVETENSKAP

protein structure

electron microscopy

transcription

Schizosaccharomyces pombe

yeast

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