Search: id:"swepub:oai:DiVA.org:kth-84671" >
Toward a Pinus pina...
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Bautista, RocíoUniversity of Malaga
(author)
Toward a Pinus pinaster bacterial artificial chromosome library
- Article/chapterEnglish2007
Publisher, publication year, extent ...
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Springer Science and Business Media LLC,2007
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:kth-84671
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-84671URI
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https://doi.org/10.1051/forest:2007060DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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QC 20120223
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Conifers are of great economic and ecological importance, but little is known concerning their genomic organization. This study is an attempt to obtain high-quality high-molecular-weight DNA from Pinus pinaster cotyledons and the construction of a pine BAC library. The preparation incorporates modifications like low centrifugation speeds, increase of EDTA concentration for plug maintenance, use of DNase inhibitors to reduce DNA degradation, use of polyvinylpyrrolidone and ascorbate to avoid secondary metabolites, and a brief electrophoresis of the plugs prior to their use. A total of 72 192 clones with an average insert size of 107 kb, which represents an equivalent of 11X pine haploid genomes, were obtained. The proportions of clones lacking inserts or containing chloroplast DNA are both approximately 1.6%. The library was screened with cDNA probes for seven genes, and two clones containing Fd-GOGAT sequences were found, one of them seemingly functional. Ongoing projects aimed at constructing a pinebacterial artificial chromosome library may benefit from the methods described here.
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Added entries (persons, corporate bodies, meetings, titles ...)
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Villalobos, David, P.University of Malaga
(author)
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Diaz-Moreno, Sara MUniversity of Malaga(Swepub:kth)u1wan3e5
(author)
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Cantón, Francisco, R.University of Malaga
(author)
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Cánovas, Francisco, M.University of Malaga
(author)
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Gonzalo Claros, M.University of Malaga
(author)
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University of Malaga
(creator_code:org_t)
Related titles
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In:Annals of Forest Science: Springer Science and Business Media LLC64:8, s. 855-8641286-45601297-966X
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