SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:kth-93008"
 

Search: onr:"swepub:oai:DiVA.org:kth-93008" > Identification of A...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Dengjel, Joern (author)

Identification of Autophagosome-associated Proteins and Regulators by Quantitative Proteomic Analysis and Genetic Screens

  • Article/chapterEnglish2012

Publisher, publication year, extent ...

  • 2012
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:kth-93008
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-93008URI
  • https://doi.org/10.1074/mcp.M111.014035DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • QC 20120411
  • Autophagy is one of the major intracellular catabolic pathways, but little is known about the composition of autophagosomes. To study the associated proteins, we isolated autophagosomes from human breast cancer cells using two different biochemical methods and three stimulus types: amino acid deprivation or rapamycin or concanamycin A treatment. The autophagosome- associated proteins were dependent on stimulus, but a core set of proteins was stimulus- independent. Remarkably, proteasomal proteins were abundant among the stimulus- independent common autophagosome- associated proteins, and the activation of autophagy significantly decreased the cellular proteasome level and activity supporting interplay between the two degradation pathways. A screen of yeast strains defective in the orthologs of the human genes encoding for a common set of autophagosome- associated proteins revealed several regulators of autophagy, including subunits of the retromer complex. The combined spatiotemporal proteomic and genetic data sets presented here provide a basis for further characterization of autophagosome biogenesis and cargo selection.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Hoyer-Hansen, Maria (author)
  • Nielsen, Maria O. (author)
  • Eisenberg, Tobias (author)
  • Harder, Lea M. (author)
  • Schandorff, Soren (author)
  • Farkas, Thomas (author)
  • Kirkegaard, Thomas (author)
  • Becker, Andrea C. (author)
  • Schroeder, Sabrina (author)
  • Vanselow, Katja (author)
  • Lundberg, EmmaKTH,Proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u12bylj1 (author)
  • Nielsen, Mogens M. (author)
  • Kristensen, Anders R. (author)
  • Akimov, Vyacheslav (author)
  • Bunkenborg, Jakob (author)
  • Madeo, Frank (author)
  • Jaattela, Marja (author)
  • Andersen, Jens S. (author)
  • KTHProteomik (creator_code:org_t)

Related titles

  • In:Molecular & Cellular Proteomics11:31535-94761535-9484

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view