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The role of compute...
The role of computer-aided design and surface chemistry on Cell-scaffold interactions
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- Yang, Sun, 1983- (författare)
- KTH,Polymerteknologi,Fiber & Polymer Technology
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- Finne-Wistrand, Anna, Asso.Prof. (preses)
- KTH,Polymerteknologi
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- Albertsson, Ann-Christine, Prof. (preses)
- KTH,Polymerteknologi
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visa fler...
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- Pan, Jinshan, Professor (opponent)
- Division of Surface and Corrosion Science, KTH
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- Hedhammar, My, Assoc. prof. (opponent)
- Dept. of Anatomy, Physiology and Biochemstry, Uppsala University
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visa färre...
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(creator_code:org_t)
- ISBN 9789175013794
- Stockholm : KTH Royal Institute of Technology, 2012
- Engelska 45 s.
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Serie: Trita-CHE-Report, 1654-1081 ; 2012:28
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Cell interactions and cell response to three dimensional scaffolds based on poly(L-lactide-co-1,5-dioxepan-2-one) [poly(LLA-co-DXO)] and poly(L-lactide-co-e-caprolactone) [poly(LLA-co-CL)] wereimprovedstudiedby increasing surface hydrophilicity and by using computer-aided scaffold design. Different amounts of Tween 80 (3, 10, 20 wt%) were mixed with the copolymer and it was found that the water contact angle decreased significantly with the amount of Tween 80. The contact angle of poly(LLA-co-CL) and poly(LLA-co-DXO) decreased from 85° to 35° and 75° to 17° respectively by using 3 wt% Tween 80. Samples with 20% Tween 80 had a water contact angle of 15°. In addition, stiffness decreased when the amount of Tween 80 was increased and thermal properties changed significantly when the amount of Tween 80 was higher than 3 wt%. Cell response was evaluated using three dimensional scaffolds and homogenous films. It was clear in this study that a high amount of Tween 80 resulting in a very low water contact angle had a toxic effect and negative influence on attachment and spreading of bone marrow stromal cells (hBMSC), while a moderate water contact angle had a positive influence on hBMSC differentiation, with significantly higher expression of Runx2 and osteocalcin. A three-dimensional fiber deposition (3DF) technique was employed to make layer-based scaffolds in a reproducible fashion. The 3DF scaffolds manufactured in this way showed enhanced mechanical properties, with E-modulus ranging from about 3 to 5 MPa in tensile strength and 0,22 to 0,29 MPa in compression tests, compared with scaffolds formed by a salt leaching method which had values of 1 and 0,03 MPa, respectively. Degradation during printing was obvious but the decrease in molecular weight did not influence thermal properties or stability of the 3DF structure. After 1 and 7 days of in vitro cell culture fluorescence staining and scanning electron microscopy showed homogeneous distribution of human osteoblasts (hOBs) on the scaffolds. The 3DF technique thus appears to be a promising method for fabrication of computer designed scaffolds as the scaffold structure is reproducible and their design can be controlled to achieve tailor made products for clinical applications.
Ämnesord
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
Nyckelord
- amorphous degradable copolymer
- aliphatic polyester
- 3D fiber deposition
- computer-aided design
- Tween 80
- BMSC
- hOB
- porous scaffolds
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