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The fatty acid amid...
The fatty acid amide hydrolase inhibitor oleoyl ethyl amide counteracts bladder overactivity in female rats
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- Gandaglia, G. (author)
- San Raffaele Scientific Institute, Milan, Italy
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- Strittmatter, F. (author)
- Lund University, Sweden
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- La Croce, G. (author)
- San Raffaele Scientific Institute, Milan, Italy,Munich University, Germany
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- Benigni, F. (author)
- San Raffaele Scientific Institute, Milan, Italy
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- Bettiga, A. (author)
- San Raffaele Scientific Institute, Milan, Italy
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- Castiglione, F. (author)
- San Raffaele Scientific Institute, Milan, Italy
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- Moschini, M. (author)
- San Raffaele Scientific Institute, Milan, Italy
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- Mistretta, F. (author)
- San Raffaele Scientific Institute, Milan, Italy
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- Gratzke, C. (author)
- Munich University, Germany
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- Montorsi, F. (author)
- San Raffaele Scientific Institute, Milan, Italy
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- Stief, C. (author)
- Munich University, Germany
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- Hedlund, Petter, 1968- (author)
- Lund University,Lunds universitet,Östergötlands Läns Landsting,Linköpings universitet,Avdelningen för läkemedelsforskning,Hälsouniversitetet,Klinisk farmakologi,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine
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(creator_code:org_t)
- 2013-08-29
- 2014
- English.
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In: Neurourology and Urodynamics. - : John Wiley & Sons. - 0733-2467 .- 1520-6777. ; 33:8, s. 1251-1258
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http://dx.doi.org/10...
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Abstract
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- AIMS:To study micturition and bladder overactivity in female rats after chronic treatment with the fatty acid amide hydrolase (FAAH) inhibitor oleoyl ethyl amide (OEtA).METHODS:Sprague-Dawley rats received daily subcutaneous injections of OEtA (0.3 mg/kg), or vehicle for 2 weeks. Cystometries, organ bath studies, Western blot, and immunofluorescence were then used. Expressions of FAAH, cannabinoid 1 and 2 receptors (CB1 and CB2), mitogen-activated protein kinase (MAPK), vesicular acetyl choline-transporter protein (VAChT), and calcitonin gene-related peptide (CGRP) were evaluated.RESULTS:At baseline, OEtA-treated rats had higher values (P < 0.05) of micturition intervals (MI) and volumes (MV), bladder capacity (BC), threshold pressure, and flow pressure than vehicle controls. Intravesical PGE2 reduced MI, MV, and BC, and increased basal pressure and the area under the curve in all rats. However, these urodynamic parameters were altered less by intravesical PGE2 in OEtA-treated rats (P < 0.05 vs. vehicle controls). Compared to vehicle controls, detrusor from OEtA-treated rats had larger contractions to carbachol at 10-0.1 µM, but no difference in Emax was recorded. FAAH, CB1, CB2, VAChT, or CGRP was similarly expressed in bladders from all rats. In separate experiments, intravesical OEtA increased mucosal expression of phosphorylated MAPK.CONCLUSIONS:Chronic FAAH inhibition altered sensory urodynamic parameters and reduced bladder overactivity. Even if it cannot be excluded that OEtA may act on central nervous sensory pathways to contribute to these effects, the presence of FAAH and CB receptors in the bladder and activation of intracellular signals for CB receptors by intravesical OEtA suggest a local role for FAAH in micturition control. Neurourol. Urodynam
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
Keyword
- cannabinoid receptor; endocannabinoid system; mitogen-activated protein kinase; urinary bladder
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Gandaglia, G.
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Strittmatter, F.
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La Croce, G.
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Benigni, F.
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Bettiga, A.
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Castiglione, F.
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show more...
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Moschini, M.
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Mistretta, F.
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Gratzke, C.
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Montorsi, F.
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Stief, C.
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Hedlund, Petter, ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Urology and Neph ...
- Articles in the publication
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Neurourology and ...
- By the university
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Linköping University
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Lund University