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Collaborative EDNAP...
Collaborative EDNAP exercise on the IrisPlex system for DNA based prediction of human eye colour
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- Chaitanya, Lakshmi (författare)
- Erasmus MC University Medical Centre Rotterdam, The Netherlands
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- Walsh, Susan (författare)
- Erasmus MC University Medical Centre Rotterdam, The Netherlands
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- Dyrberg Andersen, Jeppe (författare)
- University of Copenhagen, Denmark
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- Ansell, Ricky (författare)
- Swedish National Laboratory of Forensic Science (SKL), Linköping, Sweden
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- Ballantyne, Kaye (författare)
- Forensic Services Department, Victoria Police, Macleod, Victoria, Australia
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- Ballard, David (författare)
- School of Biomedical Sciences, King's College London, UK
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- Banemann, Regine (författare)
- Kriminaltechnik, Bundeskriminalamt, Wiesbaden, Germany
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- Maria Bauer, Christiane (författare)
- Innsbruck Medical University, Austria
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- Margarida Bento, Ana (författare)
- Instituto Nacional de Medicina Legal, Coimbra, Portugal
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- Brisighelli, Francesca (författare)
- Università Cattolica del Sacro Cuore, Rome, Italy
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- Capal, Tomas (författare)
- Institute of Criminalistics, Prague, Czech Republic
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- Clarisse, Lindy (författare)
- Netherlands Forensic Institute, The Hague, The Netherlands
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- Gross, Theresa E. (författare)
- University of Cologne, Germany
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- Haas, Cordula (författare)
- University of Zurich, Switzerland
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- Hoff-Olsen, Per (författare)
- Norwegian Institute of Public Health, Oslo, Norway
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- Hollard, Clémence (författare)
- Université de Strasbourg, Institut de Médecine Légale, France
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- Keyser, Christine (författare)
- Université de Strasbourg, Institut de Médecine Légale, France
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- Kiesler, Kevin M. (författare)
- National Institute of Standards and Technology, Gaithersburg, MD, USA
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- Kohler, Priscila (författare)
- Norwegian Institute of Public Health, Oslo, Norway
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- Kupiec, Tomasz (författare)
- Institute of Forensic Research, Kraków, Poland
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- Linacre, Adrian (författare)
- Flinders University, Adelaide, South Australia, Australia
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- Minawi, Anglika (författare)
- Kriminaltechnik, Bundeskriminalamt, Wiesbaden, Germany
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- Morling, Niels (författare)
- University of Copenhagen, Denmark
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- Nilsson, Helena (författare)
- National Board of Forensic Medicine, Linköping, Sweden
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- Norén, Lina (författare)
- Swedish National Laboratory of Forensic Science (SKL), Linköping, Sweden
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- Ottens, Renée (författare)
- Flinders University, Adelaide, South Australia, Australia
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- Palo, Jukka U. (författare)
- University of Helsinki, Finland
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- Parson, Walther (författare)
- Innsbruck Medical University, Austria
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- Pascali, Vincenzo L. (författare)
- Università Cattolica del Sacro Cuore, Rome, Italy
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- Philips, Chris (författare)
- University of Santiago de Compostela, Spain
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- João Porto, Maria (författare)
- Instituto Nacional de Medicina Legal, Coimbra, Portugal
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- Sajantila, Antti (författare)
- University of Helsinki, Finland
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- Schneider, Peter M. (författare)
- University of Cologne, Germany
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- Sijen, Titia (författare)
- Netherlands Forensic Institute, The Hague, The Netherlands
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- Söchtig, Jens (författare)
- University of Santiago de Compostela, Spain
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- Syndercombe-Court, Denise (författare)
- School of Biomedical Sciences, King's College London, UK
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- Tillmar, Andreas (författare)
- National Board of Forensic Medicine, Linköping, Sweden
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- Turanska, Martina (författare)
- Institute of Forensic Science, Slovenská Lupca, Slovakia
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- Vallone, Peter M. (författare)
- National Institute of Standards and Technology, Gaithersburg, MD, USA
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- Zatkalíková, Lívia (författare)
- Institute of Forensic Science, Slovenská Lupca, Slovakia
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- Zidkova, Anastassiya (författare)
- Charles University in Prague and General University Hospital in Prague, Czech Republic
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- Branicki, Wojciech (författare)
- Institute of Forensic Research, Kraków, Poland
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- Kayser, Manfred (författare)
- Erasmus MC University Medical Centre Rotterdam, The Netherlands
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(creator_code:org_t)
- Elsevier, 2014
- 2014
- Engelska.
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Ingår i: Forensic Science International. - : Elsevier. - 1872-4973 .- 1878-0326. ; 11, s. 241-251
- Relaterad länk:
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https://curis.ku.dk/...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The IrisPlex system is a DNA-based test system for the prediction of human eye colour from biological samples and consists of a single forensically validated multiplex genotyping assay together with a statistical prediction model that is based on genotypes and phenotypes from thousands of individuals. IrisPlex predicts blue and brown human eye colour with, on average, >94% precision accuracy using six of the currently most eye colour informative single nucleotide polymorphisms (HERC2 rs12913832, OCA2 rs1800407, SLC24A4 rs12896399, SLC45A2 (MATP) rs16891982, TYR rs1393350, and IRF4 rs12203592) according to a previous study, while the accuracy in predicting non-blue and non-brown eye colours is considerably lower. In an effort to vigorously assess the IrisPlex system at the international level, testing was performed by 21 laboratories in the context of a collaborative exercise divided into three tasks and organised by the European DNA Profiling (EDNAP) Group of the International Society of Forensic Genetics (ISFG). Task 1 involved the assessment of 10 blood and saliva samples provided on FTA cards by the organising laboratory together with eye colour phenotypes; 99.4% of the genotypes were correctly reported and 99% of the eye colour phenotypes were correctly predicted. Task 2 involved the assessment of 5 DNA samples extracted by the host laboratory from simulated casework samples, artificially degraded, and provided to the participants in varying DNA concentrations. For this task, 98.7% of the genotypes were correctly determined and 96.2% of eye colour phenotypes were correctly inferred. For Tasks 1 and 2 together, 99.2% (1875) of the 1890 genotypes were correctly generated and of the 15 (0.8%) incorrect genotype calls, only 2 (0.1%) resulted in incorrect eye colour phenotypes. The voluntary Task 3 involved participants choosing their own test subjects for IrisPlex genotyping and eye colour phenotype inference, while eye photographs were provided to the organising laboratory and judged; 96% of the eye colour phenotypes were inferred correctly across 100 samples and 19 laboratories. The high success rates in genotyping and eye colour phenotyping clearly demonstrate the reproducibility and the robustness of the IrisPlex assay as well as the accuracy of the IrisPlex model to predict blue and brown eye colour from DNA. Additionally, this study demonstrates the ease with which the IrisPlex system is implementable and applicable across forensic laboratories around the world with varying pre-existing experiences.
Nyckelord
- EDNAP
- ISFG
- IrisPlex
- Eye colour prediction
- Forensic DNA phenotyping
- FDP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
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Chaitanya, Laksh ...
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Walsh, Susan
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Dyrberg Andersen ...
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Ansell, Ricky
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Ballantyne, Kaye
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Ballard, David
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visa fler...
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Banemann, Regine
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Maria Bauer, Chr ...
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Margarida Bento, ...
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Brisighelli, Fra ...
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Capal, Tomas
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Clarisse, Lindy
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Gross, Theresa E ...
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Haas, Cordula
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Hoff-Olsen, Per
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Hollard, Clémenc ...
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Keyser, Christin ...
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Kiesler, Kevin M ...
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Kohler, Priscila
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Kupiec, Tomasz
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Linacre, Adrian
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Minawi, Anglika
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Morling, Niels
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Nilsson, Helena
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Norén, Lina
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Ottens, Renée
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Palo, Jukka U.
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Parson, Walther
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Pascali, Vincenz ...
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Philips, Chris
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João Porto, Mari ...
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Sajantila, Antti
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Schneider, Peter ...
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Sijen, Titia
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Söchtig, Jens
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Syndercombe-Cour ...
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Tillmar, Andreas
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Turanska, Martin ...
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Vallone, Peter M ...
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Zatkalíková, Lív ...
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Zidkova, Anastas ...
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Branicki, Wojcie ...
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Kayser, Manfred
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