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  • Chaitanya, LakshmiErasmus MC University Medical Centre Rotterdam, The Netherlands (författare)

Collaborative EDNAP exercise on the IrisPlex system for DNA based prediction of human eye colour

  • Artikel/kapitelEngelska2014

Förlag, utgivningsår, omfång ...

  • Elsevier,2014
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:liu-107165
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-107165URI
  • https://doi.org/10.1016/j.fsigen.2014.04.006DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • The IrisPlex system is a DNA-based test system for the prediction of human eye colour from biological samples and consists of a single forensically validated multiplex genotyping assay together with a statistical prediction model that is based on genotypes and phenotypes from thousands of individuals. IrisPlex predicts blue and brown human eye colour with, on average, >94% precision accuracy using six of the currently most eye colour informative single nucleotide polymorphisms (HERC2 rs12913832, OCA2 rs1800407, SLC24A4 rs12896399, SLC45A2 (MATP) rs16891982, TYR rs1393350, and IRF4 rs12203592) according to a previous study, while the accuracy in predicting non-blue and non-brown eye colours is considerably lower. In an effort to vigorously assess the IrisPlex system at the international level, testing was performed by 21 laboratories in the context of a collaborative exercise divided into three tasks and organised by the European DNA Profiling (EDNAP) Group of the International Society of Forensic Genetics (ISFG). Task 1 involved the assessment of 10 blood and saliva samples provided on FTA cards by the organising laboratory together with eye colour phenotypes; 99.4% of the genotypes were correctly reported and 99% of the eye colour phenotypes were correctly predicted. Task 2 involved the assessment of 5 DNA samples extracted by the host laboratory from simulated casework samples, artificially degraded, and provided to the participants in varying DNA concentrations. For this task, 98.7% of the genotypes were correctly determined and 96.2% of eye colour phenotypes were correctly inferred. For Tasks 1 and 2 together, 99.2% (1875) of the 1890 genotypes were correctly generated and of the 15 (0.8%) incorrect genotype calls, only 2 (0.1%) resulted in incorrect eye colour phenotypes. The voluntary Task 3 involved participants choosing their own test subjects for IrisPlex genotyping and eye colour phenotype inference, while eye photographs were provided to the organising laboratory and judged; 96% of the eye colour phenotypes were inferred correctly across 100 samples and 19 laboratories. The high success rates in genotyping and eye colour phenotyping clearly demonstrate the reproducibility and the robustness of the IrisPlex assay as well as the accuracy of the IrisPlex model to predict blue and brown eye colour from DNA. Additionally, this study demonstrates the ease with which the IrisPlex system is implementable and applicable across forensic laboratories around the world with varying pre-existing experiences.

Ämnesord och genrebeteckningar

  • EDNAP
  • ISFG
  • IrisPlex
  • Eye colour prediction
  • Forensic DNA phenotyping
  • FDP

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Walsh, SusanErasmus MC University Medical Centre Rotterdam, The Netherlands (författare)
  • Dyrberg Andersen, JeppeUniversity of Copenhagen, Denmark (författare)
  • Ansell, RickySwedish National Laboratory of Forensic Science (SKL), Linköping, Sweden(Swepub:liu)rican78 (författare)
  • Ballantyne, KayeForensic Services Department, Victoria Police, Macleod, Victoria, Australia (författare)
  • Ballard, DavidSchool of Biomedical Sciences, King's College London, UK (författare)
  • Banemann, RegineKriminaltechnik, Bundeskriminalamt, Wiesbaden, Germany (författare)
  • Maria Bauer, ChristianeInnsbruck Medical University, Austria (författare)
  • Margarida Bento, AnaInstituto Nacional de Medicina Legal, Coimbra, Portugal (författare)
  • Brisighelli, FrancescaUniversità Cattolica del Sacro Cuore, Rome, Italy (författare)
  • Capal, TomasInstitute of Criminalistics, Prague, Czech Republic (författare)
  • Clarisse, LindyNetherlands Forensic Institute, The Hague, The Netherlands (författare)
  • Gross, Theresa E.University of Cologne, Germany (författare)
  • Haas, CordulaUniversity of Zurich, Switzerland (författare)
  • Hoff-Olsen, PerNorwegian Institute of Public Health, Oslo, Norway (författare)
  • Hollard, ClémenceUniversité de Strasbourg, Institut de Médecine Légale, France (författare)
  • Keyser, ChristineUniversité de Strasbourg, Institut de Médecine Légale, France (författare)
  • Kiesler, Kevin M.National Institute of Standards and Technology, Gaithersburg, MD, USA (författare)
  • Kohler, PriscilaNorwegian Institute of Public Health, Oslo, Norway (författare)
  • Kupiec, TomaszInstitute of Forensic Research, Kraków, Poland (författare)
  • Linacre, AdrianFlinders University, Adelaide, South Australia, Australia (författare)
  • Minawi, AnglikaKriminaltechnik, Bundeskriminalamt, Wiesbaden, Germany (författare)
  • Morling, NielsUniversity of Copenhagen, Denmark (författare)
  • Nilsson, HelenaNational Board of Forensic Medicine, Linköping, Sweden (författare)
  • Norén, LinaSwedish National Laboratory of Forensic Science (SKL), Linköping, Sweden (författare)
  • Ottens, RenéeFlinders University, Adelaide, South Australia, Australia (författare)
  • Palo, Jukka U.University of Helsinki, Finland (författare)
  • Parson, WaltherInnsbruck Medical University, Austria (författare)
  • Pascali, Vincenzo L.Università Cattolica del Sacro Cuore, Rome, Italy (författare)
  • Philips, ChrisUniversity of Santiago de Compostela, Spain (författare)
  • João Porto, MariaInstituto Nacional de Medicina Legal, Coimbra, Portugal (författare)
  • Sajantila, AnttiUniversity of Helsinki, Finland (författare)
  • Schneider, Peter M.University of Cologne, Germany (författare)
  • Sijen, TitiaNetherlands Forensic Institute, The Hague, The Netherlands (författare)
  • Söchtig, JensUniversity of Santiago de Compostela, Spain (författare)
  • Syndercombe-Court, DeniseSchool of Biomedical Sciences, King's College London, UK (författare)
  • Tillmar, AndreasNational Board of Forensic Medicine, Linköping, Sweden (författare)
  • Turanska, MartinaInstitute of Forensic Science, Slovenská Lupca, Slovakia (författare)
  • Vallone, Peter M.National Institute of Standards and Technology, Gaithersburg, MD, USA (författare)
  • Zatkalíková, LíviaInstitute of Forensic Science, Slovenská Lupca, Slovakia (författare)
  • Zidkova, AnastassiyaCharles University in Prague and General University Hospital in Prague, Czech Republic (författare)
  • Branicki, WojciechInstitute of Forensic Research, Kraków, Poland (författare)
  • Kayser, ManfredErasmus MC University Medical Centre Rotterdam, The Netherlands (författare)
  • Erasmus MC University Medical Centre Rotterdam, The NetherlandsUniversity of Copenhagen, Denmark (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Forensic Science International: Elsevier11, s. 241-2511872-49731878-0326

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