Medium-high resolution electrochemical genotyping of HLA-DQ2/DQ8 for detection of predisposition to coeliac disease

Coeliac disease is a small intestinal disorder, induced by ingestion of gluten in genetically predisposed individuals. Coeliac disease has been strongly linked to human leukocyte antigens (HLA) located on chromosome 6, with almost 100 % of coeliac disease sufferers carrying either a HLA-DQ2 or HLA-DQ8 heterodimer, with the majority carrying HLA-DQ2 encoded by the DQA1*05:01/05:05, DQB1*02:01/02:02 alleles, whereas the remaining carry the HLA-DQ8 encoded by the DQA1*03:01, DQB1*03:02 alleles. In this work, we present the development of a multiplex electrochemical genosensor array of 36 electrodes, housed within a dedicated microfluidic platform and using a total of 10 sequence-specific probes for rapid medium-high resolution HLA-DQ2/DQ8 genotyping. An evaluation of the selectivity of the designed probes was carried out with the target sequences and 44 potentially interfering alleles, including single base mismatch differentiations; good selectivity was demonstrated. The performance of the electrochemical genosensor array was validated, analyzing real human samples for the presence of HLA-DQ2/DQ8 alleles, and compared with those obtained using laboratory-based HLA typing, and an excellent correlation was obtained.

Beni, ValerioLinköpings universitet,Biosensorer och bioelektronik,Tekniska högskolan,Universitat Rovira i Virgili, Tarragona, Spain(Swepub:liu)valbe10 (författare)
Alakkulpi, NooraFinnish Red Cross Blood Service, Helsinki, Finland (författare)
Partanen, JukkaFinnish Red Cross Blood Service, Helsinki, Finland (författare)
Lind, KristinaTATAA Biocenter AB, Göteborg, Sweden (författare)
Strömbom, LindaTATAA Biocenter AB, Göteborg, Sweden (författare)
Latta, DanielInstitut für Mikrotechnik Mainz GmbH, Germany (författare)
Höth, JulianInstitut für Mikrotechnik Mainz GmbH, Germany (författare)
Katakis, IoanisUniversitat Rovira i Virgili, Tarragona, Spain (författare)
O´Sullivan, Ciara K.Universitat Rovira i Virgili, Tarragona, Spain; Institucio Catalana de Recerca i Estudis Avançats, Barcelona, Spain (författare)
Universitat Rovira i Virgili, Tarragona, SpainBiosensorer och bioelektronik (creator_code:org_t)

Ingår i:Analytical and Bioanalytical Chemistry: Springer Berlin/Heidelberg406:12, s. 2757-27691618-26421618-2650

Av författaren/redakt...: Joda, Hamdi; Beni, Valerio; Alakkulpi, Noora; Partanen, Jukka; Lind, Kristina; Strömbom, Linda; visa fler...; Latta, Daniel; Höth, Julian; Katakis, Ioanis; O´Sullivan, Ciar ...; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.