Intracellular analysis of a lymphoblastoid cell line using H-NMR

Phillips, P.J.Departments of Chemical Engineering, University of Sydney, Australia (författare)

A complete analysis of growth and protein production characteristics of a commercially relevant animal cell line has been undertaken. The model cell line was a human lymphoblastoid which produces an IgG antibody against anti-D in blood. The batch growth characteristics of this cell line under different conditions was monitored and analysis of major nutrients and products as well as amino acids in the medium was undertaken. The internal (i.e. intracellular) concentrations of a variety of species in the cell were investigated using H-NMR of cellular extracts. This analysis enabled the quantitative determination of many amino acids, biosynthetic intermediates, secreted products and energetically important intermediates.

Barford, J.P.Departments of Chemical Engineering, University of Sydney, Australia (författare)
Harbour, C.Departments of Infectious Diseases, University of Sydney, Australia (författare)
Lundberg, PeterLinköpings universitet,Avdelningen för radiologiska vetenskaper,Hälsouniversitetet,Centrum för medicinsk bildvetenskap och visualisering, CMIV,Region Östergötland, Radiofysikavdelningen US,Departments of Biochemistry, University of Sydney, Australia(Swepub:liu)petlu11 (författare)
Departments of Chemical Engineering, University of Sydney, AustraliaDepartments of Infectious Diseases, University of Sydney, Australia (creator_code:org_t)

Ingår i:Animal Cell Technology: Basic & Applied AspectsDordrecht : Springer Verlag (Kluwer), s. 351-35897894010436639789401108485

Av författaren/redakt...: Phillips, P.J.; Barford, J.P.; Harbour, C.; Lundberg, Peter

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