A35Cl--NMR study of the singular anion-binding properties of dromedary hemoglobin

Lundberg, PeterLinköpings universitet,Avdelningen för radiologiska vetenskaper,Hälsouniversitetet,Centrum för medicinsk bildvetenskap och visualisering, CMIV,Region Östergötland, Radiofysikavdelningen US,Department of Biological Sciences, University of Calgary, Calgary Canada (författare)

0006-3002 (Print) Journal Article Research Support, Non-U.S. Gov't
35Cl(-)-NMR measurements of chloride binding to carbonmonoxy- and deoxy-dromedary hemoglobin reveal the existence of two classes of chloride-binding sites, one of high and the other of low affinity. Although this situation resembles that described for human hemoglobin, it was found that the number of binding sites as well as the association equilibrium constant for chloride binding are significantly higher in the dromedary protein. This difference may be due to the greater number of basic residues exposed to solvent and to the higher flexibility of dromedary hemoglobin. The two oxygen-linked polyanion-binding sites characteristic of this hemoglobin show competition for some of the high-affinity chloride-binding sites in keeping with their location in the cleft enclosed by the beta chains and between the alpha chains termini. It is suggested that the observed anion-binding properties of dromedary hemoglobin may contribute to the control of the physiological osmotic shock after rehydration.

Vogel, Hans J.Department of Biological Sciences, University of Calgary, Calgary Canada (författare)
Drakenberg, TorbjörnDepartment of Physical Chemistry 2, University of Lund, Lund Sweden (författare)
Forsén, StureDepartment of Physical Chemistry 2, University of Lund, Lund Sweden (författare)
Amiconi, GinoCNR Center of Molecular Biology and Department of Biochemical Sciences, Rome Italy (författare)
Forlani, LucianoDepartment of Experimental Medicine, University ‘La Sapienza’, Rome Italy (författare)
Chiancone, EmiliaCNR Center of Molecular Biology and Department of Biochemical Sciences, Rome Italy (författare)
Linköpings universitetAvdelningen för radiologiska vetenskaper (creator_code:org_t)

Ingår i:Biochimica et Biophysica Acta: Elsevier BV999:1, s. 12-80006-30021878-2434
Ingår i:Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology: Elsevier BV999:1, s. 12-80167-4838

Av författaren/redakt...: Lundberg, Peter; Vogel, Hans J.; Drakenberg, Torb ...; Forsén, Sture; Amiconi, Gino; Forlani, Luciano; visa fler...; Chiancone, Emili ...; visa färre...

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