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- Bivik, CarolineLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten (author)
Novel Genes Involved in Controlling Specification of Drosophila FMRFamide Neuropeptide Cells
- Article/chapterEnglish2015
Publisher, publication year, extent ...
- 2015-06-18
- Genetics Society of America,2015
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:liu-121318
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-121318URI
- https://doi.org/10.1534/genetics.115.178483DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
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Notes
- The expression of neuropeptides is often extremely restricted in the nervous system, making them powerful markers for addressing cell specification . In the developing Drosophila ventral nerve cord, only six cells, the Ap4 neurons, of some 10,000 neurons, express the neuropeptide FMRFamide (FMRFa). Each Ap4/FMRFa neuron is the last-born cell generated by an identifiable and well-studied progenitor cell, neuroblast 5-6 (NB5-6T). The restricted expression of FMRFa and the wealth of information regarding its gene regulation and Ap4 neuron specification makes FMRFa a valuable readout for addressing many aspects of neural development, i.e., spatial and temporal patterning cues, cell cycle control, cell specification, axon transport, and retrograde signaling. To this end, we have conducted a forward genetic screen utilizing an Ap4-specific FMRFa-eGFP transgenic reporter as our readout. A total of 9781 EMS-mutated chromosomes were screened for perturbations in FMRFa-eGFP expression, and 611 mutants were identified. Seventy-nine of the strongest mutants were mapped down to the affected gene by deficiency mapping or whole-genome sequencing. We isolated novel alleles for previously known FMRFa regulators, confirming the validity of the screen. In addition, we identified novel essential genes, including several with previously undefined functions in neural development. Our identification of genes affecting most major steps required for successful terminal differentiation of Ap4 neurons provides a comprehensive view of the genetic flow controlling the generation of highly unique neuronal cell types in the developing nervous system.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine hsv//eng
- Drosophila; CNS development; neural cell fate specification; forward genetic screening; FMRFamide
Added entries (persons, corporate bodies, meetings, titles ...)
- Bahrampour, ShahrzadLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)shaba97 (author)
- Ulvklo, CarinaLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)carul86 (author)
- Nilsson, PatrikLinköpings universitet,Institutionen för klinisk och experimentell medicin,Medicinska fakulteten(Swepub:liu)patni60 (author)
- Angel, AnnaLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)annan53 (author)
- Fransson, FredrikLinköpings universitet,Medicinska fakulteten,Avdelningen för mikrobiologi och molekylär medicin(Swepub:liu)frefr58 (author)
- Lundin, ErikaLinköpings universitet,Institutionen för klinisk och experimentell medicin,Medicinska fakulteten (author)
- Renhorn, JakobLinköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten(Swepub:liu)jakre54 (author)
- Thor, StefanLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)steth80 (author)
- Linköpings universitetAvdelningen för mikrobiologi och molekylär medicin (creator_code:org_t)
Related titles
- In:Genetics: Genetics Society of America200:4, s. 1229-12440016-67311943-2631
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