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Proteomic analyses ...
Proteomic analyses of early response of unicellular eukaryotic microorganism Tetrahymena thermophila exposed to TiO2 particles.
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- Rajapakse, K. (författare)
- University of Ljubljana, Ljubljana, Slovenia
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- Drobne, D. (författare)
- University of Ljubljana, Ljubljana, Slovenia,Jožef Stefan Institute, Ljubljana, Slovenia
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- Kastelec, D. (författare)
- University of Ljubljana, Ljubljana, Slovenia
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- Kogej, K. (författare)
- University of Ljubljana, Ljubljana, Slovenia
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- Makovec, D. (författare)
- Jožef Stefan Institute, Ljubljana, Slovenia
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- Gallampois, Christine (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
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- Amelina, H. (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm University, Stockholm, Sweden
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- Danielsson, G. (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik,Stockholm University, Stockholm, Sweden
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- Fanedl, L. (författare)
- University of Ljubljana, Ljubljana, Slovenia
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- Marinsek-Logar, R. (författare)
- University of Ljubljana, Ljubljana, Slovenia
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- Cristobal, Susana (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,IKERBASQUE, Basque Foundation for Science, University of the Basque Country, Leioa, Spain,Cristobal Lab, environmental proteomics
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(creator_code:org_t)
- 2015-11-02
- 2016
- Engelska.
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Ingår i: Nanotoxicology. - Taylor & Francis : Informa UK Limited. - 1743-5390 .- 1743-5404. ; 10:5, s. 542-556
- Relaterad länk:
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https://liu.diva-por... (primary) (Raw object)
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http://liu.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Key biological functions involved in cell survival have been studied to understand the difference between the impact of exposure to TiO2 nanoparticles (TiO2-NPs) and their bulk counterparts (bulk-TiO2). By selecting a unicellular eukaryotic model organism and applying proteomic analysis an overview of the possible impact of exposure could be obtained. In this study, we investigated the early response of unicellular eukaryotic protozoan Tetrahymena thermophila exposed to TiO2-NPs or bulk-TiO2 particles at subtoxic concentrations for this organism. The proteomic analysis based on 2DE + nLC-ESI-MS/MS revealed 930 distinct protein spots, among which 77 were differentially expressed and 18 were unambiguously identified. We identified alterations in metabolic pathways, including lipid and fatty acid metabolism, purine metabolism and energetic metabolism, as well as salt stress and protein degradation. This proteomic study is consistent with our previous findings, where the early response of T. thermophila to subtoxic concentrations of TiO2 particles included alterations in lipid and fatty acid metabolism and ion regulation. The response to the lowest TiO2-NPs concentration differed significantly from the response to higher TiO2-NPs concentration and both bulk-TiO2concentrations. Alterations on the physiological landscape were significant after exposure to both nano- and bulk-TiO2; however, no toxic effects were evidenced even at very high exposure concentrations. This study confirms the relevance of the alteration of the lipid profile and lipid metabolism in understanding the early impact of TiO2-NPs in eukaryotic cells, for example, phagocytosing cells like macrophages and ciliated cells in the respiratory epithelium.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Nanotoxicology
- particle toxicology
- proteomics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Rajapakse, K.
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Drobne, D.
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Kastelec, D.
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Kogej, K.
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Makovec, D.
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Gallampois, Chri ...
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visa fler...
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Amelina, H.
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Danielsson, G.
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Fanedl, L.
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Marinsek-Logar, ...
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Cristobal, Susan ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Cellbiologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
- Artiklar i publikationen
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Nanotoxicology
- Av lärosätet
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Linköpings universitet
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Stockholms universitet