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Protocol for Three-...
Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes
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- Bagheri, Maryam (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
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- Rezakhani, Arjang (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
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- Roghani, Mehrdad (author)
- Neurophysiology Research Center, Shahed University, Iran
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- Joghataei, Mohammad T. (author)
- Iran University of Medical Science, Iran
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- Mohseni, Simin (author)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten
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(creator_code:org_t)
- 2015-12-11
- 2015
- English.
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In: Journal of Visualized Experiments. - : JOURNAL OF VISUALIZED EXPERIMENTS. - 1940-087X. ; :106, s. e53113-
- Related links:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Subject headings
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- As glial cells in the brain, astrocytes have diverse functional roles in the central nervous system. In the presence of harmful stimuli, astrocytes modify their functional and structural properties, a condition called reactive astrogliosis. Here, a protocol for assessment of the morphological properties of astrocytes is presented. This protocol includes quantification of 12 different parameters i.e. the surface area and volume of the tissue covered by an astrocyte (astrocyte territory), the entire astrocyte including branches, cell body, and nucleus, as well as total length and number of branches, the intensity of fluorescence immunoreactivity of antibodies used for astrocyte detection, and astrocyte density (number/1,000 mu m(2)). For this purpose three-dimensional (3D) confocal microscopic images were created, and 3D image analysis software such as Volocity 6.3 was used for measurements. Rat brain tissue exposed to amyloid beta(1-40) (A beta(1-40)) with or without a therapeutic intervention was used to present the method. This protocol can also be used for 3D morphometric analysis of other cells from either in vivo or in vitro conditions.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk bildbehandling (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Image Processing (hsv//eng)
Keyword
- Neuroscience; Issue 106; Amyloid beta; Astrocyte size; Confocal microscope; Surface area; Three-dimensional morphometry; Volume
Publication and Content Type
- ref (subject category)
- art (subject category)
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