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Regulatory T-cell a...
Regulatory T-cell associated activity in Photopheresis-induced Immune tolerance in Recent Onset Type 1 Diabetes Children
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- Jonson, Carl-Oscar (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
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- Pihl, Mikael (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
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- Nyholm, Caroline (författare)
- CRC, Lund University, Department of Clinical Sciences, Malmö, Sweden
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- Cilio, Corrado (författare)
- Lund University,Lunds universitet,Diabetes - immunovirologi,Forskargrupper vid Lunds universitet,Diabetes - Immunovirology,Lund University Research Groups,CRC, Lund University, Department of Clinical Sciences, Malmö, Sweden
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- Ludvigsson, Johnny (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
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- Faresjö, Maria (författare)
- Linköpings universitet,Pediatrik,Hälsouniversitetet,Hälsouniversitet i Linköping
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- Bolmeson, Caroline (författare)
- Lund University,Lunds universitet,Create Health,Annan verksamhet, LTH,Lunds Tekniska Högskola,Evolutionär ekologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Other operations, LTH,Faculty of Engineering, LTH,Evolutionary ecology,Department of Biology,Faculty of Science
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Phil, M (författare)
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(creator_code:org_t)
- 2008-06-28
- 2008
- Engelska.
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Ingår i: Clinical and Experimental Immunology. - : Oxford University Press (OUP). - 0009-9104 .- 1365-2249. ; 153:2, s. 174-181
- Relaterad länk:
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http://urn.kb.se/res...
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http://dx.doi.org/10...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- Extracorporeal photochemotherapy (ECP) has demonstrated immunological effects. The proposed cytotoxic lymphocyte antigen 4 (CTLA-4) involvement, together with forkhead box P3 (FoxP3) and transforming growth factor (TGF)-β are associated with regulatory T cell activity. The aim of the study was to evaluate the regulatory T cell-associated effect of ECP in recent onset type 1 diabetic (T1D) children. Children (n = 20) with T1D received photopheresis 8-methoxypsoralen + ECP or placebo + shampheresis. Peripheral blood mononuclear cells (PBMC) collected pretreatment (day 1) and post-treatment (day 90) were stimulated with phytohaemagglutinin (PHA) and T1D-associated glutamic acid decarboxylase 65 (GAD65) peptide a.a. 247–279. CTLA-4, sCTLA-4, FoxP3 and TGF-β mRNA transcription was quantified. Photopheresis-treated individuals' relative mRNA expression was generally maintained during the course of the study. Placebo individuals increased in spontaneous CTLA-4 mRNA (P < 0·05) but decreased in expression after stimulation with GAD65-peptide (P < 0·05) and PHA (P < 0·05). Spontaneous TGF-β (P < 0·05) increased whereas PHA- (P < 0·01) and GAD65-peptide (P < 0·01)-induced TGF-β expression decreased in the placebo group, whereas it was maintained in the treated group. Without intervention, expression of CTLA-4 and TGF-β, stimulated with PHA and GAD65 peptide, decreased with time, with a parallel reduction of GAD65-peptide and PHA-stimulated TGF-β expression. These parameters were counteracted by ECP. In conclusion, our results indicate that ECP maintains regulatory T cell-associated activity in recent-onset T1D.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- children
- extracorporeal photochemotherapy
- T1D
- Treg
- MEDICINE
- MEDICIN
- T1D
- T-reg
- children
- extracorporeal photochemotherapy
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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