Positive MRI Enhancement in THP-1 Cells with Gd2O3 Nanoparticles

Klasson, AnnaLinköpings universitet,Medicinsk radiologi,Hälsouniversitetet,Centrum för medicinsk bildvetenskap och visualisering, CMIV (author)

There is a demand for more efficient and tissue-specific MRI contrast agents and recent developments involve the design of substances useful as molecular markers and magnetic tracers. In this study, nanoparticles of gadolinium oxide (Gd2O3) have been investigated for cell labeling and capacity to generate a positive contrast. THP-1, a monocytic cell line that is phagocytic, was used and results were compared with relaxivity of particles in cell culture medium (RPMI 1640). The results showed that Gd2O3-labeled cells have shorter T1 and T2 relaxation times compared with untreated cells. A prominent difference in signal intensity was observed, indicating that Gd2O3 nanoparticles can be used as a positive contrast agent for cell labeling. The r1 for cell samples was 4.1 and 3.6 s-1 mm-1 for cell culture medium. The r2 was 17.4 and 12.9 s-1 mm-1, respectively. For r1, there was no significant difference in relaxivity between particles in cells compared to particles in cell culture medium, (pr1 = 0.36), but r2 was significantly different for the two different series (pr2 = 0.02). Viability results indicate that THP-1 cells endure treatment with Gd2O3 nanoparticles for an extended period of time and it is therefore concluded that results in this study are based on viable cells.

Ahrén, MariaLinköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten(Swepub:liu)marah49 (author)
Hellqvist, EvaLinköpings universitet,Cellbiologi,Hälsouniversitetet(Swepub:liu)evahe25 (author)
Söderlind, FredrikLinköpings universitet,Fysikalisk Kemi,Tekniska fakulteten(Swepub:liu)freso40 (author)
Rosén, AndersLinköpings universitet,Cellbiologi,Tekniska högskolan(Swepub:liu)andro72 (author)
Käll, Per-OlovLinköpings universitet,Fysikalisk Kemi,Tekniska högskolan(Swepub:liu)perka28 (author)
Uvdal, KajsaLinköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten(Swepub:liu)kajuv09 (author)
Engström, MariaLinköpings universitet,Medicinsk radiologi,Tekniska högskolan,Centrum för medicinsk bildvetenskap och visualisering, CMIV(Swepub:liu)maren21 (author)
Linköpings universitetMedicinsk radiologi (creator_code:org_t)

By the author/editor: Klasson, Anna; Ahrén, Maria; Hellqvist, Eva; Söderlind, Fredr ...; Rosén, Anders; Käll, Per-Olov; show more...; Uvdal, Kajsa; Engström, Maria; show less...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.