Effect of several antioxidants on thawed ram spermatozoa submitted to 37°C up to four hours.

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Mata-Campuzano, MaríaITRA-ULE, INDEGSAL, University of León, León, Spain; Molecular Biology (Cell Biology), University of León, Spain (författare)

Effect of several antioxidants on thawed ram spermatozoa submitted to 37°C up to four hours.

Artikel/kapitelEngelska2012

Förlag, utgivningsår, omfång ...

2012-02-28
Blackwell Verlag,2012
printrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:liu-130192
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-130192URI
https://doi.org/10.1111/j.1439-0531.2012.01990.xDOI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

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Thawed ram spermatozoa were incubated at 37°C in the presence of dehydroascorbic acid (DHA), TEMPOL (TPL), N-acetyl-cysteine (NAC) and rutin (RUT), at 0.1 and 1 mm, in order to test their effects on sperm physiology. Cryopreserved spermatozoa from four rams were thawed, pooled, washed and incubated in TALP-Hepes with 1 mm or 0.1 mm of each antioxidant, performing a replicate with induced oxidative stress (Fe(2+) /ascorbate). Motility (CASA), viability and mitochondrial membrane potential (flow cytometry) were analysed at 2 and 4 h. Lipoperoxidation (MDA production), intracellular reactive oxygen species (ROS) and DNA status (TUNEL) were analysed at 4 h. Antioxidants, except DHA 0.1 mm, decreased motility and kinematic parameters, but had little effect on viability or mitochondrial activity. Except 1 mm DHA, the antioxidants reduced ROS at 4 h. Moreover, NAC 1 mm, rutin and TEMPOL reduced ROS and DNA damage in the presence of oxidative stress. N-acetyl-cysteine, rutin 1 mm and TEMPOL reduced lipoperoxidation in the presence of oxidative stress. However, DHA did not affect lipoperoxidation. At 1 mm, DHA increased DNA damage in the absence of oxidative stress. Dehydroascorbic acid effects could arise from spermatozoa having a low capacity for reducing it to ascorbic acid, and it may be tested in the presence of other antioxidants or reducing power. Future research should focus in testing whether the inhibition of motility observed for NAC, rutin and TEMPOL is reversible. These antioxidants might be useful at lower temperatures (refrigerated storage or cryopreservation) when their protective effects could be advantageous.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Utvecklingsbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Developmental Biology hsv//eng

Biuppslag (personer, institutioner, konferenser, titlar ...)

Alvarez-Rodríguez, ManuelITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, Spain(Swepub:liu)manal80 (författare)
Alvarez, MercedesITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, Spain (författare)
Anel, LuisITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, Spain (författare)
de Paz, PaulinoITRA-ULE, INDEGSAL, University of León, León, Spain; Molecular Biology (Cell Biology), University of León, Spain (författare)
Garde, JulianBiology of Reproduction Group, National Wildlife Research Institute (IREC) (UCLM-CSIC-JCCM), Albacete, Spain (författare)
Martínez-Pastor, FelipeITRA-ULE, INDEGSAL, University of León, León, Spain; Molecular Biology (Cell Biology), University of León, Spain (författare)
ITRA-ULE, INDEGSAL, University of León, León, Spain; Molecular Biology (Cell Biology), University of León, SpainITRA-ULE, INDEGSAL, University of León, León, Spain; Animal Reproduction and Obstetrics, University of León, Spain (creator_code:org_t)

Sammanhörande titlar

Ingår i:Reproduction in domestic animals: Blackwell Verlag47:6, s. 907-9140936-67681439-0531

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