A SNP panel for identity and kinship testing using massive parallel sequencing

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Grandell, IdaNational Board Forens Med, Department Forens Genet and Forens Toxicol, Artillerigatan 12, SE-58758 Linkoping, Sweden (författare)

A SNP panel for identity and kinship testing using massive parallel sequencing

Artikel/kapitelEngelska2016

Förlag, utgivningsår, omfång ...

2016-03-01
SPRINGER,2016
printrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:liu-130267
https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-130267URI
https://doi.org/10.1007/s00414-016-1341-4DOI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:art swepub-publicationtype

Anmärkningar

Within forensic genetics, there is still a need for supplementary DNA marker typing in order to increase the power to solve cases for both identity testing and complex kinship issues. One major disadvantage with current capillary electrophoresis (CE) methods is the limitation in DNA marker multiplex capability. By utilizing massive parallel sequencing (MPS) technology, this capability can, however, be increased. We have designed a customized GeneRead DNASeq SNP panel (Qiagen) of 140 previously published autosomal forensically relevant identity SNPs for analysis using MPS. One single amplification step was followed by library preparation using the GeneRead Library Prep workflow (Qiagen). The sequencing was performed on a MiSeq System (Illumina), and the bioinformatic analyses were done using the software Biomedical Genomics Workbench (CLC Bio, Qiagen). Forty-nine individuals from a Swedish population were genotyped in order to establish genotype frequencies and to evaluate the performance of the assay. The analyses showed to have a balanced coverage among the included loci, and the heterozygous balance showed to have less than 0.5 % outliers. Analyses of dilution series of the 2800M Control DNA gave reproducible results down to 0.2 ng DNA input. In addition, typing of FTA samples and bone samples was performed with promising results. Further studies and optimizations are, however, required for a more detailed evaluation of the performance of degraded and PCR-inhibited forensic samples. In summary, the assay offers a straightforward sample-to-genotype workflow and could be useful to gain information in forensic casework, for both identity testing and in order to solve complex kinship issues.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Annan medicin och hälsovetenskap Rättsmedicin hsv//swe
MEDICAL AND HEALTH SCIENCES Other Medical and Health Sciences Forensic Science hsv//eng
Next generation sequencing; Single-nucleotide polymorphism; Forensic genetics; Human identification; Kinship

Biuppslag (personer, institutioner, konferenser, titlar ...)

Samara, RaedQIAGEN Science Inc, MD 21703 USA (författare)
Tillmar, AndreasLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten,National Board Forens Med, Department Forens Genet and Forens Toxicol, Artillerigatan 12, SE-58758 Linkoping, Sweden(Swepub:liu)andka62 (författare)
National Board Forens Med, Department Forens Genet and Forens Toxicol, Artillerigatan 12, SE-58758 Linkoping, SwedenQIAGEN Science Inc, MD 21703 USA (creator_code:org_t)

Sammanhörande titlar

Ingår i:International journal of legal medicine: SPRINGER130:4, s. 905-9140937-98271437-1596

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Av författaren/redakt...: Grandell, Ida; Samara, Raed; Tillmar, Andreas

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Annan medicin oc ...; och Rättsmedicin

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Av lärosätet: Linköpings universitet

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