Vaginal Lactobacillus flora of healthy Swedish women

Vasquez, AlejandraLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine,Laboratory of Food Hygiene, Division of Food Technology, Lund University, Lund, Sweden (author)

Species of the Lactobacillus acidophilus complex are generally considered to constitute most of the vaginal Lactobacillus flora, but the flora varies between studies. However, this may be due to difficulties in identifying the closely related species within the L. acidophilus complex by using traditional methods and to variations in the vaginal status of the participants. Two hundred two isolates from the vaginal fluids of 23 Swedish women without bacterial vaginosis, as defined by the criteria of Nugent et al. (R. P. Nugent, M. A. Krohn, and S. L. Hillier, J. Clin. Microbiol. 29:297-301, 1991), were typed by randomly amplified polymorphic DNA (RAPD) analysis and identified to the species level by temporal temperature gradient gel electrophoresis, multiplex PCR, and 16S ribosomal DNA sequencing. The vaginal flora of most participants was dominated by a single RAPD type, but five of them harbored two RAPD types representing two different species or strains. The most frequently occurring species were Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus iners, and Lactobacillus jensenii. L. iners has not previously been reported as one of the predominant Lactobacillus species in the vagina.

Jakobsson, TellLinköpings universitet,Obstetrik och gynekologi,Hälsouniversitetet(Swepub:liu)telja07 (author)
Ahrné, SivLund University,Lunds universitet,Livsmedelsteknik,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Food Technology, Engineering and Nutrition,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH,Division of Food Technology, Lund University, Lund(Swepub:lu)livt-sah (author)
Forsum, UrbanÖstergötlands Läns Landsting,Linköpings universitet,Klinisk mikrobiologi,Hälsouniversitetet(Swepub:liu)urbfo84 (author)
Molin, GöranLund University,Lunds universitet,Livsmedelsteknik,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Food Technology, Engineering and Nutrition,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH,Division of Food Technology, Lund University, Lund, Sweden(Swepub:lu)livt-gmo (author)
Avdelningen för medicinsk mikrobiologiInstitutionen för laboratoriemedicin (creator_code:org_t)

By the author/editor: Vasquez, Alejand ...; Jakobsson, Tell; Ahrné, Siv; Forsum, Urban; Molin, Göran

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

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