Fluorescence in situ hybridization markers for prediction of cervical lymph node metastases.

Wangsa, DarawaleeDepartment of Oncology-Pathology, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden. Genetics Branch, National Institutes of Health, Bethesda, Maryland (författare)

The presence of lymph node metastases is associated with poor prognosis in early stage cervical cancer. As of yet, no molecular markers predicting lymph node metastases have been identified. We examined single genetic markers and a composite marker, comprised of three fluorescence in situ hybridization (FISH) probes targeting the genes LAMP3, PROX1, and PRKAA1, in pretreatment cervical biopsies from 16 lymph node positive cases and 15 lymph node negative controls from women with stage IB and IIA cervical cancer. In addition, we determined clonal patterns by including CCND1 to compare the clonal constitution of primary tumors and associated lymph node metastases. The composite FISH marker allowed for classification of patients into those with and without lymph node metastases with a sensitivity and specificity of 75% and 87%, respectively (P = 0.001). The positive predictive value and negative predictive value were 86% and 76%, respectively. Clonal patterns varied among the tumors. In many cases, changes between the primary tumor and lymph node metastases in the most common clones may indicate that certain clones have a growth advantage for establishing metastases in lymph nodes. We conclude that the composite FISH marker may be useful for determining risk for subsequent development of lymph node metastases in patients with cervical cancer.

Heselmeyer-Haddad, KerstinGenetics Branch, National Institutes of Health, Bethesda, Maryland (författare)
Ried, PatriciaDepartment of Oncology-Pathology, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden (författare)
Eriksson, ElinaKarolinska Institutet (författare)
Schäffer, Alejandro ACenter for Cancer Research, National Cancer Institute, National Center for Biotechnology Information, National Institutes of Health, Bethesda, Maryland (författare)
Morrison, Larry EAbbott Molecular, Inc, Des Plaines, Illinois (författare)
Luo, JuhuaSchool of Social Development and Public Policy, Beijing Normal University, Beijing, China (författare)
Auer, GertKarolinska Institutet (författare)
Munck-Wikland, EvaDepartment of Oto-Rhino-Laryngology, Head and Neck Surgery, Radiumhemmet, Karolinska University Hospital, Stockholm, Sweden (författare)
Ried, ThomasGenetics Branch, National Institutes of Health, Bethesda, Maryland (författare)
Åvall Lundqvist, ElisabethDepartment of Gynaecologic Oncology, Radiumhemmet, Karolinska University Hospital, Stockholm, Sweden(Swepub:liu)eliav51 (författare)
Department of Oncology-Pathology, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden. Genetics Branch, National Institutes of Health, Bethesda, MarylandGenetics Branch, National Institutes of Health, Bethesda, Maryland (creator_code:org_t)

Ingår i:American Journal of Pathology: Elsevier175:6, s. 2637-26450002-94401525-2191

Av författaren/redakt...: Wangsa, Darawale ...; Heselmeyer-Hadda ...; Ried, Patricia; Eriksson, Elina; Schäffer, Alejan ...; Morrison, Larry ...; visa fler...; Luo, Juhua; Auer, Gert; Munck-Wikland, E ...; Ried, Thomas; Åvall Lundqvist, ...; visa färre...

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