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  • Peev, D.University of Nebraska Lincoln, NE 68588 USA (author)

Anisotropic contrast optical microscope

  • Article/chapterEnglish2016

Publisher, publication year, extent ...

  • AMER INST PHYSICS,2016
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:liu-133895
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-133895URI
  • https://doi.org/10.1063/1.4965878DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • An optical microscope is described that reveals contrast in the Mueller matrix images of a thin, transparent, or semi-transparent specimen located within an anisotropic object plane (anisotropic filter). The specimen changes the anisotropy of the filter and thereby produces contrast within the Mueller matrix images. Here we use an anisotropic filter composed of a semi-transparent, nanostructured thin film with sub-wavelength thickness placed within the object plane. The sample is illuminated as in common optical microscopy but the light is modulated in its polarization using combinations of linear polarizers and phase plate (compensator) to control and analyze the state of polarization. Direct generalized ellipsometry data analysis approaches permit extraction of fundamental Mueller matrix object plane images dispensing with the need of Fourier expansion methods. Generalized ellipsometry model approaches are used for quantitative image analyses. These images are obtained from sets of multiple images obtained under various polarizer, analyzer, and compensator settings. Up to 16 independent Mueller matrix images can be obtained, while our current setup is limited to 11 images normalized by the unpolarized intensity. We demonstrate the anisotropic contrast optical microscope by measuring lithographically defined micro-patterned anisotropic filters, and we quantify the adsorption of an organic self-assembled monolayer film onto the anisotropic filter. Comparison with an isotropic glass slide demonstrates the image enhancement obtained by our method over microscopy without the use of an anisotropic filter. In our current instrument, we estimate the limit of detection for organic volumetric mass within the object plane of approximate to 49 fg within approximate to 7 x 7 mu m(2) object surface area. Compared to a quartz crystal microbalance with dissipation instrumentation, where contemporary limits require a total load of approximate to 500 pg for detection, the instrumentation demonstrated here improves sensitivity to a total mass required for detection by 4 orders of magnitude. We detail the design and operation principles of the anisotropic contrast optical microscope, and we present further applications to the detection of nanoparticles, to novel approaches for imaging chromatography and to new contrast modalities for observations on living cells. Published by AIP Publishing.

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  • Hofmann, TinoLinköpings universitet,Halvledarmaterial,Tekniska fakulteten,University of Nebraska Lincoln, NE 68588 USA; University of North Carolina Charlotte, NC 28223 USA(Swepub:liu)tinho73 (author)
  • Kananizadeh, N.University of Nebraska Lincoln, NE 68588 USA (author)
  • Beeram, S.University of Nebraska Lincoln, NE 68588 USA (author)
  • Rodriguez, E.University of Nebraska Lincoln, NE 68588 USA (author)
  • Wimer, S.University of Nebraska Lincoln, NE 68588 USA (author)
  • Rodenhausen, K. B.Biolin Science Inc, NJ 07652 USA (author)
  • Herzinger, C. M.JA Woollam Co Inc, NE 68508 USA (author)
  • Kasputis, T.University of Michigan, MI 48109 USA (author)
  • Pfaunmiller, E.Celerion Inc, NE 68502 USA (author)
  • Nguyen, A.University of Nebraska Lincoln, NE 68583 USA (author)
  • Korlacki, R.University of Nebraska Lincoln, NE 68588 USA (author)
  • Pannier, A.University of Nebraska Lincoln, NE 68588 USA (author)
  • Li, Y.University of Nebraska Lincoln, NE 68588 USA (author)
  • Schubert, E.University of Nebraska Lincoln, NE 68588 USA (author)
  • Hage, D.University of Nebraska Lincoln, NE 68588 USA (author)
  • Schubert, MathiasLinköpings universitet,Halvledarmaterial,Tekniska fakulteten,University of Nebraska Lincoln, NE 68588 USA; Leibniz Institute Polymer Research IPF Dresden, Germany(Swepub:liu)schma39 (author)
  • University of Nebraska Lincoln, NE 68588 USAHalvledarmaterial (creator_code:org_t)

Related titles

  • In:Review of Scientific Instruments: AMER INST PHYSICS87:110034-67481089-7623

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