Probe for simultaneous membrane and nucleus labeling in living cells and in vivo bioimaging using a two-photon absorption water-soluble Zn(II) terpyridine complex with a reduced pi-conjugation system

Zhang, Qian (author): Linköpings universitet,Ytors Fysik och Kemi,Tekniska fakulteten,Anhui University, Peoples R China

Uvdal, Kajsa (author): Linköpings universitet,Molekylär ytfysik och nanovetenskap,Tekniska fakulteten

Tian, Yupeng (author): Anhui University, Peoples R China; Nanjing University, Peoples R China

(creator_code:org_t)

2017
2017
English.
In: Chemical Science. - : ROYAL SOC CHEMISTRY. - 2041-6520 .- 2041-6539. ; 8:1, s. 142-149

Related links:: https://liu.diva-por... (primary) (Raw object); show more...; https://pubs.rsc.org...; https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Abstract Subject headings

Small, biocompatible and water-soluble molecules with high two-photon absorption (2PA) cross-section values (delta) are in high demand for specific bioimaging applications. Here, two novel terpyridine derivative ligands with donor-acceptor (D-A) (L1) and donor-pi-acceptor (D-pi-A) (L2) models, and their corresponding Zn(II) complexes are designed and characterized. It was found that the two-photon absorption cross section values (d) in the near-infrared region (NIR, about 800 nm) are significantly enhanced for complexes 1 and 2 compared to their free D-A type ligand L1, while those of complexes 3 and 4 were greatly decreased relative to their free ligand L2, thus confirming that the smaller ligand (D-A type) displays a suitable Turn-ON fluorescence pair for two-photon fluorescence microscopy (2PFM). Firstly, the potential of simultaneously labeling a live cell plasma membrane and nucleus using complex 1 is demonstrated. In addition, live larval and adult zebrafish incubated with an optimal concentration of 1 demonstrated clear brain uptake. Lastly and importantly, using such a probe to visualize the blood-brain- barrier (BBB) capillary endothelial cells and penetrate the BBB into the central nervous system (CNS) intravenously in a mouse model is also explored.

By the author/editor: Tian, Xiaohe; Zhang, Qian; Zhang, Mingzhu; Uvdal, Kajsa; Wang, Qin; Chen, Junyang; show more...; Du, Wei; Huang, Bei; Wu, Jieying; Tian, Yupeng; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Organic Chemistr ...

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