Sökning: WFRF:(Jin Bin) >
Inflammation and Ap...
Inflammation and Apoptosis: Dual Mediator Role for Toll-like Receptor 4 in the Development of Necrotizing Enterocolitis
-
- Zhou, Yin (författare)
- Sichuan University, Peoples R China
-
- Li, Yibo (författare)
- Sichuan University, Peoples R China
-
- Zhou, Bin (författare)
- Sichuan University, Peoples R China
-
visa fler...
-
- Chen, Keling (författare)
- Sichuan University, Peoples R China
-
- Lyv, Zhaoying (författare)
- Sichuan University, Peoples R China
-
- Huang, Dongmei (författare)
- Sichuan University, Peoples R China
-
- Liu, Bin (författare)
- Sichuan University, Peoples R China
-
- Xu, Zhicheng (författare)
- Sichuan University, Peoples R China
-
- Xiang, Bo (författare)
- Sichuan University, Peoples R China
-
- Jin, Shuguang (författare)
- Sichuan University, Peoples R China
-
- Sun, Xiao-Feng, 1959- (författare)
- Linköpings universitet,Avdelningen för Kirurgi, Ortopedi och Onkologi,Medicinska fakulteten,Region Östergötland, Onkologiska kliniken US
-
- Li, Yuan (författare)
- Sichuan University, Peoples R China
-
visa färre...
-
(creator_code:org_t)
- LIPPINCOTT WILLIAMS & WILKINS, 2017
- 2017
- Engelska.
-
Ingår i: Inflammatory Bowel Diseases. - : LIPPINCOTT WILLIAMS & WILKINS. - 1078-0998 .- 1536-4844. ; 23:1, s. 44-56
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: Necrotizing enterocolitis (NEC) is the leading cause of neonatal gastrointestinal mortality; effective interventions are lacking with limited understanding of the pathogenesis of NEC. The importance of Toll-like receptor 4 (TLR4) signaling in NEC is well documented; however, the potential mechanisms that regulate enterocyte inflammation and apoptosis remain unclear. The aim of this study was to characterize the role of TLR4-mediated inflammation and apoptosis in the development of NEC and to determine the major apoptotic pathways and regulators in the process. Methods: TLR4-deficient C57BL/10ScNJ mice and lentivirus-mediated stable TLR4-silent cell line (IEC-6) were used. NEC was induced by formula gavage, cold, hypoxia, combined with lipopolysaccharide in vivo or lipopolysaccharide stimulation in vitro. Enterocyte apoptosis was evaluated by TUNEL or Annexin analysis. The expression of TLR4, caspase3, caspase8, caspase9, Bip, Bax, Bcl-2, and RIP was detected by Western blot and immunofluorescence. Inflammatory factors such as tumor necrosis factor-a and interleukin-2 were examined by Luminex. Results: Defect of TLR4 led to suppressed enterocytes apoptosis both in vitro and in vivo; the expression of caspase3, caspase8, Bip, and Bax was decreased; and caspase9 and Bcl-2 were increased. NEC severity was attenuated in TLR4-deficient mice compared with wild-type counterparts, and enterocytes apoptosis was correlated with NEC severity. RIP and cytokine level of tumor necrosis factor-a and interleukin-2 were also decreased. Conclusions: TLR4-induced inflammation and apoptosis play a critical role in the pathogenesis of NEC. TLR4 inhibition, combined with extrinsic (caspase8) and/or endoplasmic reticulum stress (Bip) apoptosis signaling blockade could serve as a potential effective treating strategy for NEC.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
Nyckelord
- necrotizing enterocolitis; Toll-like receptor 4; intestinal inflammation; apoptosis; necrosis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Zhou, Yin
-
Li, Yibo
-
Zhou, Bin
-
Chen, Keling
-
Lyv, Zhaoying
-
Huang, Dongmei
-
visa fler...
-
Liu, Bin
-
Xu, Zhicheng
-
Xiang, Bo
-
Jin, Shuguang
-
Sun, Xiao-Feng, ...
-
Li, Yuan
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Gastroenterologi
- Artiklar i publikationen
-
Inflammatory Bow ...
- Av lärosätet
-
Linköpings universitet