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Amperometric L-argi...
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Zhybak, Mykhailo T.National Academic Science Ukraine, Ukraine
(författare)
Amperometric L-arginine biosensor based on a novel recombinant arginine deiminase
- Artikel/kapitelEngelska2017
Förlag, utgivningsår, omfång ...
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2017-05-02
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SPRINGER WIEN,2017
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-139518
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-139518URI
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https://doi.org/10.1007/s00604-017-2290-4DOI
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Funding Agencies|European Community [PIRSES-GA-2012-318053]; NATO Science for Peace (SFP) [CBP.NUKR.SFPP 984173]
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The authors describe an amperometric biosensor for the amino acid L-arginine (L-Arg). It is based on the use of a Nafion/Polyaniline (PANi) composite on a platinum screen-printed electrode (Pt-SPE) using a novel recombinant arginine deiminase isolated from Mycoplasma hominis. The protein was over-expressed, purified and employed as a biorecognition element of the sensor. Enzymatic hydrolysis of L-Arg leads to the formation of ammonium ions which diffuse into the Nafion/PANi layer and induce the electroreduction of PANi at a potential of -0.35 V (vs Ag/AgCl). L-Arg sensitivity is 684 +/- 32 A.M-1.m(-2), and the apparent Michaelis-Menten constant K-M(app)) is 0.31 +/- 0.05 mM. The calibration plot is linear over the range 3-200 mu M L-Arg, the limit of detection is 1 mu M, and the response time (for 90% of the total signal change to occur) is 15 s. The sensor is selective and exhibits good storage stability (amp;gt; 1 month without loss in signal). The biosensor was applied to the analysis of L-Arg in pharmaceutical samples and of ammonium and L-Arg in spiked human plasma obtained from blood of healthy volunteers and those with a hepatic disorder. Data generated were found to be in good agreement with a reference fluorometric enzymatic assay.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Fayura, Lyubov Y.NAS Ukraine, Ukraine
(författare)
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Boretsky, Yuriy R.Lviv State University of Phys Culture, Ukraine
(författare)
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Gonchar, Mykhailo V.NAS Ukraine, Ukraine
(författare)
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Sibirny, Andriy A.NAS Ukraine, Ukraine; Rzeszow University, Poland
(författare)
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Dempsey, EithneITT Dublin, Ireland
(författare)
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Turner, AnthonyLinköpings universitet,Biosensorer och bioelektronik,Tekniska fakulteten(Swepub:liu)anttu13
(författare)
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Korpan, Yaroslav I.National Academic Science Ukraine, Ukraine
(författare)
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National Academic Science Ukraine, UkraineNAS Ukraine, Ukraine
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Microchimica Acta: SPRINGER WIEN184:8, s. 2679-26860026-36721436-5073
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